Cloning, Characterization, and FISH Mapping of Four Satellite DNAs from Black Muntjac (Muntiacus crinifrons) and Fea's Muntjac (M. feae)
文献类型:期刊论文
作者 | Liu Y1,4; Nie WH1; Huang L1,4; Wang JH1; Su WT1; Lin Chyi-C3; Yang FT[*]1,2 |
刊名 | 动物学研究
![]() |
出版日期 | 2008 |
卷号 | 29期号:3页码:225—235 |
关键词 | FISH mapping Satellite DNA Muntiacus Tandem chromosome fusion |
其他题名 | 黑麂和费氏麂四种卫星DNA的克隆特征和染色体定位 |
通讯作者 | fy1@sanger.ac.uk |
合作状况 | 其它 |
中文摘要 | 近年来,分子细胞遗传学研究已基本证实了染色体的串联融合(端粒-着丝粒融合)是麂属动物核型演化的主要重排方式。尽管染色体串联融合的分子机制还不清楚,但通过染色体的非同源重组,着丝粒区域的卫星DNA被认为可能介导了染色体的融合。以前的研究发现在赤麂和小麂染色体的大部分假定的串联融合位点处存在着非随机分布的卫星DNA。然而在麂属的其他物种中,这些卫星DNA的组成以及在基因组中的分布情况尚未被研究。本研究从黑麂和费氏麂基因组中成功地克隆了4种卫星DNA(BMC5、BM700、BM1.1k和FM700),并分析了这些卫星克隆的特征以及在小麂、黑麂、贡山麂和费氏麂染色体上的定位情况。结果表明,卫星I和IIDNA(BMC5,BM700和FM700)的信号除了分布在这些麂属动物染色体的着丝粒区域外,也间隔地分布在这些物种的染色体臂上。其研究结果为黑麂、费氏麂和贡山麂的染色体核型也是从一个2n=70的共同祖先核型通过一系列的串联融合进化而来的假说提供了直接的证据。 |
英文摘要 | Recent molecular cytogenetic studies demonstrate that extensive centromere-telomere fusions are the main chromosomal rearrangements underlying the karyotypic evolution of extant muntjacs. Although the molecular mechanism of tandem fusions remains unknown, satellite DNA is believed to have facilitated chromosome fusions by non-allelic homologous recombination. Previous studies detected non-random hybridization signals of cloned satellite DNA at the postulated fusion sites on the chromosomes in Indian and Chinese muntjacs. But the genomic distribution and organization of satellite DNAs in other muntjacs have not been investigated. In this study, we have isolated four satellite DNA clones (BMCS, BM700, BM 1.1 k and FM700) from the black muntjac (Muntiacus crinifrons) and Fea's muntjac (M. feae), and hybridized these four clones onto chromosomes of four muntjac species (M. reevesi, M. crinifrons, M. gongshanenisis and M. feae). Besides the predominant centromeric signals, non-random interstitial hybridization signals from satellite I and II DNA clones (BMC5, BM700 and FM700) were also observed on the arms of chromosomes of these four muntjacs. Our results provide additional support for the notion that the karyotypes of M. crinifrons, M. feae and M. gongshanensis have evolved from a 2n = 70 ancestral karyotype by a series of chromosome fusions. |
收录类别 | 其他 |
资助信息 | Supported partly by the grant from the National Natural Science Foundation of China (30270719) |
语种 | 英语 |
公开日期 | 2010-08-05 |
源URL | [http://159.226.149.42:8088/handle/152453/927] ![]() |
专题 | 昆明动物研究所_保护遗传学(细胞库) 昆明动物研究所_遗传资源与进化国家重点实验室 |
作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming Yunnan 650223,China 2.Wellcome Trust Sanger Institute;Wellcome Trust Genome Campus;Hinxton;Cambridge;UK 3.Department of Medical Research, China Medical University Hospital, Taichung Taiwan 404,China 4.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China |
推荐引用方式 GB/T 7714 | Liu Y,Nie WH,Huang L,等. Cloning, Characterization, and FISH Mapping of Four Satellite DNAs from Black Muntjac (Muntiacus crinifrons) and Fea's Muntjac (M. feae)[J]. 动物学研究,2008,29(3):225—235. |
APA | Liu Y.,Nie WH.,Huang L.,Wang JH.,Su WT.,...&Yang FT[*].(2008).Cloning, Characterization, and FISH Mapping of Four Satellite DNAs from Black Muntjac (Muntiacus crinifrons) and Fea's Muntjac (M. feae).动物学研究,29(3),225—235. |
MLA | Liu Y,et al."Cloning, Characterization, and FISH Mapping of Four Satellite DNAs from Black Muntjac (Muntiacus crinifrons) and Fea's Muntjac (M. feae)".动物学研究 29.3(2008):225—235. |
入库方式: OAI收割
来源:昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。