Molecular cloning, characterization and localization of chicken type II procollagen gene
文献类型：期刊论文
| 作者 | Xi CX1; Liu N1; Liang F1; Guo SQ1; Sun YY1; Yang FT2; Xi YZ[*]1 |
| 刊名 | GENE
 |
| 出版日期 | 2006 |
| 卷号 | 366期号:1页码:67-76 |
| 关键词 | genomic organization splicing developmental dynamics evolutional characters expression |
| ISSN号 | 0378-1119 |
| 通讯作者 | xiyz@yahoo.com |
| 合作状况 | 其它 |
| 英文摘要 | Chicken type II procollagen (ccol2a1) has become as an important oral tolerance protein for effective treatment of rheumatoid arthritis. However, its molecular identity remains unclear. Here, we reported the full-length cDNA and nearly complete genomic DNA encoding ccol2a1. We have determined the structural organization, evolutional characters, developmental expression and chromosomal mapping of the gene. The full-length cDNA sequence spans 4837 by containing all the coding region of the ccol2a1 including 3' and 5' untranslation region. The deduced peptide of ccol2a1, composed of 1420 amino acids, can be divided into signal peptide, N-propeptide, N-telopeptide, triple helix, C-telopeptide and C-propeptide. The ccol2a1 genomic DNA sequence was determined to be 12,523 by long containing 54 exons interrupted by 53 introns. Comparison of the ccol2a1 with its counterparts in human, mouse, canine, horse, rat, frog and newt revealed highly conserved sequence in the triple helix domain. Chromosomal mapping of ccol2a1 locates it on 4P2. While the ccol2al mRNA was expressed in multiple tissues, the protein was only detected in chondrogenic cartilage, vitreous body and cornea. The ccol2al was found to contain two isoforms detected by RT-PCR. The distribution of the ccol2a1 lacking exon 2 was frequently detected in chondrogenic tissues, whereas the exon 2-containing isoform was more abundant in non-chondrogenic tissues. These results provide useful information for preparing recombinant chicken type II collagen and for a better understanding of normal cartilage development. |
| 收录类别 | SCI |
| 资助信息 | This study was supported by grants from National Natural Sciences Foundation of China (No. 30430290). |
| 原文出处 | 200636667.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2045]  |
| 专题 | 昆明动物研究所_其他 |
| 作者单位 | 1.Department of Immunology and National Center for Biomedicine Analysis, Beijing 307 Hospital Affiliated to Academy of Medical Sciences, No. 8 Dong-Da Street, Beijing, 100071, P. R. China 2.Kuming Institute of Zoology, Chinese Academy of Sciences, Kuming, P.R. China |
| 推荐引用方式 GB/T 7714 | Xi CX,Liu N,Liang F,et al. Molecular cloning, characterization and localization of chicken type II procollagen gene[J]. GENE,2006,366(1):67-76. |
| APA | Xi CX.,Liu N.,Liang F.,Guo SQ.,Sun YY.,...&Xi YZ[*].(2006).Molecular cloning, characterization and localization of chicken type II procollagen gene.GENE,366(1),67-76. |
| MLA | Xi CX,et al."Molecular cloning, characterization and localization of chicken type II procollagen gene".GENE 366.1(2006):67-76. |
入库方式： OAI收割
来源：昆明动物研究所
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