Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting
文献类型：期刊论文
| 作者 | Nie WH[*]1; Wang JH1; Su WT1; Wang YX1; Yang FT2 |
| 刊名 | CHROMOSOME RESEARCH
 |
| 出版日期 | 2009 |
| 卷号 | 17期号:3页码:321-329 |
| 关键词 | pangolin Manis fluorescence in-situ hybridization chromosome painting chromosome rearrangement karyotype cytogenetics |
| ISSN号 | 0967-3849 |
| 通讯作者 | whnie@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | The Chinese pangolin (Manis pentadactyla), a representative species of the order Pholidota, has been enlisted in the mammalian whole-genome sequencing project mainly because of its phylogenetic importance. Previous studies showed that the diploid number of M. pentadactyla could vary from 2n = 36 to 42. To further characterize the genome organization of M. pentadactyla and to elucidate chromosomal mechanism underlying the karyotype diversity of Pholidota, we flow-sorted the chromosomes of 2n = 40 M. pentadactyla, and generated a set of chromosome-specific probes by DOP-PCR amplification of flow-sorted chromosomes. A comparative chromosome map between M. pentadactyla and the Malayan pangolin (Manis javanica, 2n = 38), as well as between human and M. pentadactyla, was established by chromosome painting for the first time. Our results demonstrate that seven Robertsonian rearrangements, together with considerable variations in the quantity of heterochromatin and in the number of nucleolar organizer regions (NORs) differentiate the karyotypes of 2n = 38 M. javanica and 2n = 40 M. pentadactyla. Moreover, we confirm that the M. javanica Y chromosome bears one NOR. Comparison of human homologous segment associations found in the genomes of M. javanica and M. pentadactyla revealed seven shared associations (HSA 1q/11, 2p/5, 2q/10q, 4p+q/20, 5/13, 6/19p and 8q/10p) that could constitute the potential Pholidota-specific signature rearrangements. |
| 收录类别 | SCI |
| 资助信息 | This study is supported partly by the grant from the National Natural Science Foundation of China (No. 30770293). |
| 原文出处 | 2009173321.pdf |
| 语种 | 英语 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/2059]  |
| 专题 | 昆明动物研究所_保护遗传学(细胞库) 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_其他 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, P. R. China 2.Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK |
| 推荐引用方式 GB/T 7714 | Nie WH[*],Wang JH,Su WT,et al. Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting[J]. CHROMOSOME RESEARCH,2009,17(3):321-329. |
| APA | Nie WH[*],Wang JH,Su WT,Wang YX,&Yang FT.(2009).Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting.CHROMOSOME RESEARCH,17(3),321-329. |
| MLA | Nie WH[*],et al."Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting".CHROMOSOME RESEARCH 17.3(2009):321-329. |
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来源：昆明动物研究所
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