间接免疫荧光鉴别奶牛胚胎性别的研究
文献类型：期刊论文
| 作者 | 陈云鹤; 门红升; 周瑆良; 佴文惠; 和协超; 杨怀林; 杨建珍 |
| 刊名 | 动物学研究
 |
| 出版日期 | 1993 |
| 卷号 | 14期号:3页码:246-250 |
| 关键词 | H—Y抗原 小鼠胚胎 性别鉴别 免疫荧光 |
| ISSN号 | 0254-5853 |
| 其他题名 | Study on Sex Identification of Bovine preimplantation Embryos by Indirect Immunofluorescent Assay |
| 中文摘要 | 以间接免疫荧光法检测昆明系小鼠早期胚胎雄性特异性H—Y抗原的表达。结果表明:54%的胚胎为H—Y阳性(雄性);46%为H—Y阴性(雌性)。经与昆明系小鼠的自然性比率(♂:52%;♀:48%)比较,两者无显著性差异。 经细胞遗传学方法确证,以免疫荧光法鉴别的胚胎性别,雄性的鉴别准确率为75.50%;雌性为82.97%。 本文还就影响鉴别准确率的若干因素以及本方法在生产中的应用前景等问题进行了分析讨论。 |
| 英文摘要 | Totally 37 normal 6-7 days old bovine embryos collected from superovulated cows were used in this study.Embryos were first stained in 1:5 diluted H-Y antisera in M2 for 30 minutes and subsequently in 1:10 diluted fluorescencein isothiocynate-conjugated goat anti-mouse IgG for another 30 minutes.Both stain procedures were performed under 39℃,5%CO2,95% air in incubator.Or these embryos,57% (21/37) with fluorescence were classified as H-Y negative (male) and 43% (16/37) without fluorescence as H-Y negative (female) under inverted fluorescence microscope.There was no significant difference between our result and bovine natural sex ratio (P<0.05).Five fluorescence positive embryos and three fluorescence negative embryos were transferred to 5 recipients (one or two embryos per recipient),two healthy female calve were born by H-Y negative embryo bearing recipients arter full term in vivo development.Out result indicated that this method in sex identification are reliable. |
| 收录类别 | 其他 |
| 资助信息 | 国家863生物技术领域基金;国家自然科学基金 |
| 原文出处 | 19931403246.pdf |
| 语种 | 中文 |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/3289]  |
| 专题 | 昆明动物研究所_哺乳动物胚胎发育 昆明动物研究所_保护遗传学(细胞库) 昆明动物研究所_认知障碍病理学 昆明动物研究所_生殖与发育生物学 |
| 作者单位 | 中国科学院昆明动物研究所;昆明 650107 |
| 推荐引用方式 GB/T 7714 | 陈云鹤,门红升,周瑆良,等. 间接免疫荧光鉴别奶牛胚胎性别的研究[J]. 动物学研究,1993,14(3):246-250. |
| APA | 陈云鹤.,门红升.,周瑆良.,佴文惠.,和协超.,...&杨建珍.(1993).间接免疫荧光鉴别奶牛胚胎性别的研究.动物学研究,14(3),246-250. |
| MLA | 陈云鹤,et al."间接免疫荧光鉴别奶牛胚胎性别的研究".动物学研究 14.3(1993):246-250. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。