Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise
文献类型：期刊论文
| 作者 | Wang JZ1; Su WT2; Nie WH2; Wang JH2; Xiao WH[*]1; Wang D[*]1 |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
 |
| 出版日期 | 2011 |
| 卷号 | 47期号:9页码:618-630 |
| 关键词 | Finless porpoise Fibroblast cell line Co-transfection In vitro |
| 通讯作者 | w-xiao@ihb.ac.cn ; wangd@ihb.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | The Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis), as the sole freshwater subspecies of N. phocaenoides, is endemic to the Yangtze River and its adjacent lakes. Its population has declined significantly over recent decades. In this study, we established a skin-derived finite fibroblast cell line of the Yangtze finless porpoise, named YFP-SF1, using primary cell culture methods, and an immortalized cell line, T-YFP-SF1, through co-transfection (GFP and SV40 T antigens) techniques. YFP-SF1 proliferated continuously with a minimum population doubling time of 31 h and exhibited age-dependent changes in growth rate. T-YFP-SF1 cells exhibited fibroblast morphology and were characterized by a shorter doubling time, higher attachment efficiencies, colony formation at a low seeding density, and growth in low serum concentrations. Anchorage independence and foci formation in the cell monolayer were observed from passage 36. The chromosome number of YFP-SF1 and T-YFP-SF1 remained stable at 2n=44 in the early passages, and the viability of thawed cells remained above 90% after cryopreservation in liquid nitrogen. Taken together, we have established fibroblast cell lines of Yangtze finless porpoise for the first time, which might assist as an in vitro model for this endangered mammal |
| 收录类别 | SCI |
| 资助信息 | This research was supported in part by the National Key Basic Research Foundation (973 Project; no. 2007CB411600), the National Natural Science Foundation of China (no. 30730018), and the Open Project of State Key Laboratory of Genetic Resources and Evolution (GREKF08-06). |
| 语种 | 英语 |
| 公开日期 | 2012-03-16 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6866]  |
| 专题 | 昆明动物研究所_保护遗传学(细胞库) 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Key Laboratory of Biodiversity and Conservation of Aquatic Organisms, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, People’s Republic of China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, People’s Republic of China |
| 推荐引用方式 GB/T 7714 | Wang JZ,Su WT,Nie WH,et al. Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2011,47(9):618-630. |
| APA | Wang JZ,Su WT,Nie WH,Wang JH,Xiao WH[*],&Wang D[*].(2011).Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,47(9),618-630. |
| MLA | Wang JZ,et al."Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 47.9(2011):618-630. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。