Karyotype analysis of mithun (Bos frontalis) and mithun bull x Brahman cow hybrids
文献类型：期刊论文
| 作者 | Qu KX1,2,3; He ZX2; Nie WH4; Zhang JC2; Jin XD2; Yang GR2; Yuan XP2; Huang BZ2; Zhang YP3,4; Zan LS[*]1,5 |
| 刊名 | GENETICS AND MOLECULAR RESEARCH
 |
| 出版日期 | 2012 |
| 卷号 | 11期号:1页码:131-140 |
| 关键词 | Karyotype Rob(2 Mithun (Bos frontalis) 28) Brahman cow (Bos indicus) Hybrids of mithun x Brahman |
| 通讯作者 | zanls@yahoo.com.cn |
| 合作状况 | 其它 |
| 英文摘要 | We examined the cytogenetics of mithun (Bos frontalis), domesticated version of the Asian gaur, and hybrids (F-1 generation) produced by artificial insemination of Brahman cows (Bos indicus) with mithun semen. Reproductive potential was also examined in the F1 generation and a backcrossed heifer for utilization of heterosis. Metaphase chromosome spreads were examined by conventional staining and fluorescence in situ hybridization hybridized with the entire chromosome 1 of mithun as a specific probe. Chromosome 1 of mithun was found to be equivalent to Bos taurus chromosomes 2 and 28. The karyotype of the female mithun (N = 4) comprised 58 chromosomes, including 54 acrocentric and four large submetacentric chromosomes, without the four acrocentric chromosomes found in the domesticated species B. indicus. However, one of the four female mithuns with a normal mithun phenotype had an abnormal karyotype (2n = 59), indicating introgression from B. taurus or B. indicus. The F-1 karyotypes (N = 6, 3 male 3 female) of the mithun bull x Brahman cow cross had 2n = 59, intermediate between their parents; they were consistent heterozygous carriers with a centric fusion involving rob(2;28), as expected. Two pronounced red signals were seen in the mithun karyotypes, three red signals in the mithun x Brahman hybrids, and four red signals in the Brahman cattle, in good agreement with centric fusion of bovine rob(2;28). The female backcross hybrid (N = 1) with 2n = 59 had a similar chromosome configuration to the F-1 karyotypes and had rob(2;28). Such female backcross hybrids normally reproduce; however, the F-1 bulls (N = 3) had not yet generated normal sperm at 24 months |
| 收录类别 | SCI |
| 资助信息 | Research supported by the Sci-Tech Tackle Key Project and High-Tech Development Program of the Yunnan Province (#2006NG23), the General Program of Applied Basic Re- search of the Yunnan Province (#2006C0068M), the Sci-Tech Tackle Key Project of the Yun- nan Province (#2008LA018), the National “863” Program of China (#2008AA101010), the Special Plan of Public Sector of Ministry of Agriculture, China (#NYHYZX07-035), and the Technological Innovation and Training Innovative Talents Program of the Yunnan Province (#2010CI082). |
| 语种 | 英语 |
| WOS记录号 | WOS:000301923500014 |
| 公开日期 | 2012-04-20 |
| 源URL | [http://159.226.149.42:8088/handle/152453/6922]  |
| 专题 | 昆明动物研究所_分子进化基因组学 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_保护遗传学(细胞库) |
| 作者单位 | 1.College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, China 2.Yunnan Academy of Grassland and Animal Science, Kunming, Yunnan, China 3.Yunnan Key Laboratory of Molecular Biology of Domestic Animal and Laboratory of Molecular Evolution and Genome Diversity, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 5.National Beef Cattle Improvement Centre of China, Yangling, Shaanxi, China |
| 推荐引用方式 GB/T 7714 | Qu KX,He ZX,Nie WH,et al. Karyotype analysis of mithun (Bos frontalis) and mithun bull x Brahman cow hybrids[J]. GENETICS AND MOLECULAR RESEARCH,2012,11(1):131-140. |
| APA | Qu KX.,He ZX.,Nie WH.,Zhang JC.,Jin XD.,...&Zan LS[*].(2012).Karyotype analysis of mithun (Bos frontalis) and mithun bull x Brahman cow hybrids.GENETICS AND MOLECULAR RESEARCH,11(1),131-140. |
| MLA | Qu KX,et al."Karyotype analysis of mithun (Bos frontalis) and mithun bull x Brahman cow hybrids".GENETICS AND MOLECULAR RESEARCH 11.1(2012):131-140. |
入库方式： OAI收割
来源：昆明动物研究所
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