朱鹮细胞系的建立及其生物学特性观察
文献类型：期刊论文
| 作者 | 王金焕1; 苏榕2; 苏伟婷1; 成诚2; 魏曙光2; 丁海华3; 段英3; 李生斌2; 佴文惠[*]1 |
| 刊名 | 动物学研究
 |
| 出版日期 | 2012 |
| 卷号 | 33期号:6页码:591-596 |
| 关键词 | 朱鹮 细胞培养 生长特征 染色体带型 |
| 其他题名 | Establishment of cell lines derived from skin biopsies of crested ibis (Nipponia nippon) and their biological characteristics. |
| 通讯作者 | whnie@mail.kiz.ac.cn |
| 中文摘要 | 濒危级生物朱鹮是朱鹮属的唯一物种。为保藏朱鹮的遗传资源并为开展其相关生物学研究提供方便的实验材料,利用采自雌、雄两只成年朱鹮的皮肤样品,成功建立了两株朱鹮的细胞系。两株细胞系的形态均为典型的成纤维样细胞;免疫荧光染色检测显示细胞强表达平滑肌特异α-actin,表明两株细胞均来源于血管平滑肌组织;生长特性分析显示细胞增殖能力在10代以前较强,在15代以后逐渐减弱,25代以后则生长缓慢,难以生成单层细胞;染色体分析显示,两株细胞系均为正常二倍体,核型为2n=68,ZZ(♂),ZW(♀)。该研究首次报道了朱鹮染色体的Ag带、C带和G带,并通过带型分析和荧光原位杂交鉴别了朱鹮的性染色体。该文建系所用的皮肤组织块贴壁种植法,可以应用于其他鸟类,特别是珍稀濒危鸟类细胞系的建立。 |
| 英文摘要 | The crested ibis is among the rarest and most endangered species worldwide. To preserve its genetic resources and conveniently provide materials for biological research, we successfully established two cell lines from biopsies of a male and female adult crested ibis. The cultured cells from both specimens had typical fibroblast morphology. Immunofluorescence staining revealed that the cultured cells strongly expressed the marker of smooth muscle specific α-actin, clearly indicating the cells were from the smooth muscle tissue. Growth property analysis showed that the cells grew well past the first 10 passages and continued growing with reduced proliferation after 15 passages, but ceased by passage 25 as the cells could not grow to form a confluent monolayer. From these two cell lines, we harvested mitotic metaphase chromosomes and conducted different staining, banding, and fluorescent in situ hybridization. Throughout the process, cells maintained normal diploidy, with the karyotypes of these two cell lines being 2n=68, ZZ in the male and 2n=68, ZW in the female. Patterns of Ag staining, C- and G-bands of the crested ibis chromosomes were also studied. Banding analyses and fluorescent in situ hybridization also allowed identification of the sex chromosomes. We suggest that the external implants method for establishing primary cell lines used in this study may also be applicable to other birds, especially similarly endangered avian species. |
| 收录类别 | 其他 |
| 资助信息 | 国家实验细胞平台专项运行经费;朱鹮野化放飞工程(2008,2010);中国科学院昆明动物研究所遗传资源与进化国家重点实验室专项经费;西安交通大学高等教育创新基金;光华教育基金(2010822). |
| 语种 | 中文 |
| 公开日期 | 2013-02-26 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7151]  |
| 专题 | 昆明动物研究所_保护遗传学(细胞库) 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.中国科学院昆明动物研究所 遗传资源与进化国家重点实验室, 云南 昆明 650223 2.西安交通大学 法医学院, 陕西西安 710061 3.国家林业总局, 陕西省林业厅, 陕西西安710082 |
| 推荐引用方式 GB/T 7714 | 王金焕,苏榕,苏伟婷,等. 朱鹮细胞系的建立及其生物学特性观察[J]. 动物学研究,2012,33(6):591-596. |
| APA | 王金焕.,苏榕.,苏伟婷.,成诚.,魏曙光.,...&佴文惠[*].(2012).朱鹮细胞系的建立及其生物学特性观察.动物学研究,33(6),591-596. |
| MLA | 王金焕,et al."朱鹮细胞系的建立及其生物学特性观察".动物学研究 33.6(2012):591-596. |
入库方式： OAI收割
来源：昆明动物研究所
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