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Sequencing and automated whole-genome optical mapping of the genome of a domestic goat (Capra hircus)
文献类型：期刊论文
| 作者 | Dong Y1,2; Xie M3; Jiang Y1,5; Xiao NQ10; Du XY4; Zhang WG1,6; Tosser-Klopp G7; Wang JH1; Yang S3; Liang J3 |
| 刊名 | NATURE BIOTECHNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 31期号:2页码:135-141 |
| 通讯作者 | xuxun@genomics.cn ; shzhao@mail.hzau.edu.cn ; wangj@genomics.cn ; wwang@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | We report the similar to 2.66-Gb genome sequence of a female Yunnan black goat. The sequence was obtained by combining short-read sequencing data and optical mapping data from a high-throughput whole-genome mapping instrument. The whole-genome mapping data facilitated the assembly of super-scaffolds >5x longer by the N50 metric than scaffolds augmented by fosmid end sequencing (scaffold N50 = 3.06 Mb, super-scaffold N50 = 16.3 Mb). Super-scaffolds are anchored on chromosomes based on conserved synteny with cattle, and the assembly is well supported by two radiation hybrid maps of chromosome 1. We annotate 22,175 protein-coding genes, most of which were recovered in the RNA-seq data of ten tissues. Comparative transcriptomic analysis of the primary and secondary follicles of a cashmere goat reveal 51 genes that are differentially expressed between the two types of hair follicles. This study, whose results will facilitate goat genomics, shows that whole-genome mapping technology can be used for the de novo assembly of large genomes. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the key project of CAS (KSZD-EW-Z-005), Chinese 973 programs (2007CB815700), the National Natural Science Foundation of China (30960246 and 31260538), the Shenzhen Municipal Government and the Yantian District local government of Shenzhen, and a CAS-Max Planck Society Fellowship and the 100 talent program of CAS to W.W. and Jun W. |
| 语种 | 英语 |
| WOS记录号 | WOS:000315322100024 |
| 公开日期 | 2013-03-21 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7284]  |
| 专题 | 昆明动物研究所_基因起源组 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_保护遗传学(细胞库) 昆明动物研究所_分子进化基因组学 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.University of Chinese Academy of Sciences, Beijing, China 3.BGI-Shenzhen, Shenzhen, China 4.Huazhong Agricultural University, Wuhan, China 5.5 CSIRO Livestock Industries, St. Lucia, Australia 6.Inner Mongolia Agricultural University, Hohhot, China 7.INRA Laboratoire de Génétique Cellulaire, Castanet-Tolosan, France 8.Utah State University, Logan, Utah, USA 9.Texas A&M University, College Station, Texas, USA 10.OpGen, Inc., Gaithersburg, Maryland, USA |
| 推荐引用方式 GB/T 7714 | Dong Y,Xie M,Jiang Y,et al. Sequencing and automated whole-genome optical mapping of the genome of a domestic goat (Capra hircus)[J]. NATURE BIOTECHNOLOGY,2013,31(2):135-141. |
| APA | Dong Y.,Xie M.,Jiang Y.,Xiao NQ.,Du XY.,...&Zhao SH[*].(2013).Sequencing and automated whole-genome optical mapping of the genome of a domestic goat (Capra hircus).NATURE BIOTECHNOLOGY,31(2),135-141. |
| MLA | Dong Y,et al."Sequencing and automated whole-genome optical mapping of the genome of a domestic goat (Capra hircus)".NATURE BIOTECHNOLOGY 31.2(2013):135-141. |
入库方式： OAI收割
来源：昆明动物研究所
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