A First Generation Comparative Chromosome Map between Guinea Pig (Cavia porcellus) and Humans
文献类型：期刊论文
| 作者 | Romanenko SA[*]1,2; Perelman PL1,2; Trifonov VA1,2; Serdyukova NA1; Li TL3; Fu BY4; O'Brien PCM5; Ng BL4; Nie WH3; Liehr T6 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2015 |
| 卷号 | 10期号:5页码:e0127937 |
| 通讯作者 | rosa@mcb.nsc.ru ; fy1@sanger.ac.uk |
| 合作状况 | 其它 |
| 英文摘要 | The domesticated guinea pig, Cavia porcellus (Hystricomorpha, Rodentia), is an important laboratory species and a model for a number of human diseases. Nevertheless, genomic tools for this species are lacking; even its karyotype is poorly characterized. The guinea pig belongs to Hystricomorpha, a widespread and important group of rodents; so far the chromosomes of guinea pigs have not been compared with that of other hystricomorph species or with any other mammals. We generated full sets of chromosome-specific painting probes for the guinea pig by flow sorting and microdissection, and for the first time, mapped the chromosomal homologies between guinea pig and human by reciprocal chromosome painting. Our data demonstrate that the guinea pig karyotype has undergone extensive rearrangements: 78 synteny-conserved human autosomal segments were delimited in the guinea pig genome. The high rate of genome evolution in the guinea pig may explain why the HSA7/16 and HSA16/19 associations presumed ancestral for eutherians and the three syntenic associations (HSA1/10, 3/19, and 9/11) considered ancestral for rodents were not found in C. porcellus. The comparative chromosome map presented here is a starting point for further development of physical and genetic maps of the guinea pig as well as an aid for genome assembly assignment to specific chromosomes. Furthermore, the comparative mapping will allow a transfer of gene map data from other species. The probes developed here provide a genomic toolkit, which will make the guinea pig a key species to unravel the evolutionary biology of the Hystricomorph rodents |
| 收录类别 | SCI |
| 资助信息 | This study was funded in part by programs MCB and SB RAS Programs and research grants of the Russian Fund for Basic Research. BF, BLN and FY were supported by Wellcome Trust (grant number WT098051), RS was supported by PRIN grant 2012, MURST, ThL and VATwere supported by DFG (LI 820/32-1). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9188]  |
| 专题 | 昆明动物研究所_保护遗传学(细胞库) 昆明动物研究所_遗传资源与进化国家重点实验室 |
| 作者单位 | 1.Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk, Russia 2.Novosibirsk State University, Novosibirsk, Russia 3.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, PR China 4.Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom 5.Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom 6.Jena University Hospital, Institute of Human Genetics and Anthropology, Jena, Germany 7.Department of Biology, University of Florence, Florence, Italy |
| 推荐引用方式 GB/T 7714 | Romanenko SA[*],Perelman PL,Trifonov VA,et al. A First Generation Comparative Chromosome Map between Guinea Pig (Cavia porcellus) and Humans[J]. PLOS ONE,2015,10(5):e0127937. |
| APA | Romanenko SA[*].,Perelman PL.,Trifonov VA.,Serdyukova NA.,Li TL.,...&Yang FT[*].(2015).A First Generation Comparative Chromosome Map between Guinea Pig (Cavia porcellus) and Humans.PLOS ONE,10(5),e0127937. |
| MLA | Romanenko SA[*],et al."A First Generation Comparative Chromosome Map between Guinea Pig (Cavia porcellus) and Humans".PLOS ONE 10.5(2015):e0127937. |
入库方式： OAI收割
来源：昆明动物研究所
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