Characterization of glial-restricted precursors from rhesus monkey embryonic stem cells
文献类型：期刊论文
| 作者 | Chen HW[*]1,3,4,7; Mao Y2,7; Li J6; Ma YY2; Wang SF1,3,7; Li B1,3,7; Wang JH5 |
| 刊名 | TRANSLATIONAL NEUROSCIENCE
 |
| 出版日期 | 2015 |
| 卷号 | 6期号:1页码:244-251 |
| 关键词 | Differentiation Glial-restricted precursor (GRP) Rhesus monkey Embryonic stem cell (ESC) |
| 通讯作者 | chenhw02@hotmail.com |
| 英文摘要 | Glial-restricted precursor (GRP) cells, the earliest glial progenitors for both astrocytes and oligodendrocytes, have been derived from embryos and embryonic stem cells (ESC) in rodents. However, knowledge regarding the equivalent cell type in primates is limited due to restrictions imposed by ethics and resources. Here we report successful derivation and characterization of primate GRP cells from rhesus monkey ESC. The purified monkey GRP cells were A(2)B(5)-positive and FGF2-dependent for survival and proliferation. The differentiation assays indicated that they were tri-potential in vitro and bi-potential in vivo. These newly purified GRP cells will help to facilitate understanding of the molecular mechanism of glial development in primates as well as provide a source of therapeutic donor cells for use in neuroregenerative medicine. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by research grants from the National Basic Research Program 2006CB701505 and 2007CB947701, the Chinese Academy of Sciences KSCX2-YW-R-47, Chinese National Science Foundation 30570906 and 30700159. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10344]  |
| 专题 | 昆明动物研究所_保护遗传学(细胞库) 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_认知障碍病理学 昆明动物研究所_其他 管理、支撑部门_大型仪器中心 |
| 作者单位 | 1.Laboratory of Reproductive and Developmental Biology, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China 2.Laboratory of Primate Recognition Neurosciences, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China 3.Yunnan Key Laboratory of Animal Reproductive Biology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China 4.Stem Cell and Brain Research Institute, INSERM U846, Bron 69675, France 5.Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China 6.Central Laboratory, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R. China 7.Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China |
| 推荐引用方式 GB/T 7714 | Chen HW[*],Mao Y,Li J,et al. Characterization of glial-restricted precursors from rhesus monkey embryonic stem cells[J]. TRANSLATIONAL NEUROSCIENCE,2015,6(1):244-251. |
| APA | Chen HW[*].,Mao Y.,Li J.,Ma YY.,Wang SF.,...&Wang JH.(2015).Characterization of glial-restricted precursors from rhesus monkey embryonic stem cells.TRANSLATIONAL NEUROSCIENCE,6(1),244-251. |
| MLA | Chen HW[*],et al."Characterization of glial-restricted precursors from rhesus monkey embryonic stem cells".TRANSLATIONAL NEUROSCIENCE 6.1(2015):244-251. |
入库方式： OAI收割
来源：昆明动物研究所
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