Construction, characterization and chromosomal mapping of bacterial artificial chromosome (BAC) library of Yunnan snub-nosed monkey (Rhinopithecus bieti)
文献类型：期刊论文
| 作者 | Xu HL1,3,4; Qian YP2; Su B[*]1,2; Nie WH1; Chi JX1,3; Yang FT1 |
| 刊名 | CHROMOSOME RESEARCH
 |
| 出版日期 | 2004 |
| 卷号 | 12期号:3页码:251-262 |
| 关键词 | bacterial artificial chromosome (BAC) chromosome mapping fluorescence in-situ hydridization Rhinopithecus bieti Yunnan snub-nosed monkey |
| 通讯作者 | sub@mail.kiz.ac.cn ; bing.su@uc.edu |
| 合作状况 | 其它 |
| 英文摘要 | We constructed a high redundancy bacterial artificial chromosome library of a seriously endangered Old World Monkey, the Yunnan snub-nosed monkey (Rhinopithecus bieti) from China. This library contains a total of 136 320 BAC clones. The average insert size of BAC clones was estimated to be 148 kb. The percentage of small inserts (50-100 kb) is 2.74%, and only 2.67% non-recombinant clones were observed. Assuming a similar genome size with closely related primate species, the Yunnan snub-nosed monkey BAC library has at least six times the genome coverage. By end sequencing of randomly selected BAC clones, we generated 201 sequence tags for the library. A total of 139 end-sequenced BAC clones were mapped onto the chromosomes of Yunnan snub-nosed monkey by fluorescence in-situ hybridization, demonstrating a high degree of synteny conservation between humans and Yunnan snub-nosed monkeys. Blast search against human genome showed a good correlation between the number of hit clones and the size of the chromosomes, an indication of unbiased chromosomal distribution of the BAC library. This library and the mapped BAC clones will serve as a valuable resource in comparative genomics studies and large-scale genome sequencing of nonhuman primates. The DNA sequence data reported in this paper were deposited in GenBank and assigned the accession number CG891489-CG891703. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the Chinese Academy of Sciences (CAS) and the National Science Foundation of China (NSFC). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/10619]  |
| 专题 | 昆明动物研究所_保护遗传学(细胞库) 昆明动物研究所_比较基因组学 昆明动物研究所_细胞与分子进化重点实验室 |
| 作者单位 | 1.Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, China 2.Center for Genome Information, Department of Environmental Health, University of Cincinnati, OH, USA 3.Graduate School of the Chinese Academy of Sciences, Beijing, China 4.College of Animal Science and Technology, Sichuan Agricultural University, Yaan, China |
| 推荐引用方式 GB/T 7714 | Xu HL,Qian YP,Su B[*],et al. Construction, characterization and chromosomal mapping of bacterial artificial chromosome (BAC) library of Yunnan snub-nosed monkey (Rhinopithecus bieti)[J]. CHROMOSOME RESEARCH,2004,12(3):251-262. |
| APA | Xu HL,Qian YP,Su B[*],Nie WH,Chi JX,&Yang FT.(2004).Construction, characterization and chromosomal mapping of bacterial artificial chromosome (BAC) library of Yunnan snub-nosed monkey (Rhinopithecus bieti).CHROMOSOME RESEARCH,12(3),251-262. |
| MLA | Xu HL,et al."Construction, characterization and chromosomal mapping of bacterial artificial chromosome (BAC) library of Yunnan snub-nosed monkey (Rhinopithecus bieti)".CHROMOSOME RESEARCH 12.3(2004):251-262. |
入库方式： OAI收割
来源：昆明动物研究所
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