3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum
文献类型：期刊论文
| 作者 | Xiao, Shiyuan1,2; Xu, Jingliang1 ; Chen, Xiaoyan1; Li, Xiekun1,2; Zhang, Yu1; Yuan, Zhenhong1
|
| 刊名 | molecular biotechnology
 |
| 出版日期 | 2016-05-01 |
| 卷号 | 58期号:5页码:311-318 |
| 关键词 | 3-Methyl-1-butanol Corynebacterium glutamicum alpha-Ketoisovalerate decarboxylase Alcohol dehydrogenase Gene knockout |
| 英文摘要 | biofuel offers a promising solution to the adverse environmental problems and depletion in reserves of fossil fuels. higher alcohols including 3-methyl-1-butanol were paid much more attention as fuel substitute in recent years, due to its similar properties to gasoline. in the present work, 3-methyl-1-butanol production in engineered corynebacterium glutamicum was studied. alpha-ketoisovalerate decarboxylase gene (kivd) from lactococcus lactis combined with alcohol dehydrogenase gene (adh2, adha, and adh3) from three organisms were overexpressed in c. glutamicum. enzymatic assay and alcohol production results showed that adh3 from zymomonas mobilis was the optimum candidate for 3-methyl-1-butanol production in c. glutamicum. the recombinant with kivd and adh3 could produce 0.182 g/l of 3-methyl-1-butanol and 0.144 g/l of isobutanol after 12 h of incubation. further inactivation of the e1 subunit of pyruvate dehydrogenase complex gene (acee) and lactic dehydrogenase gene (ldh) in the above c. glutamicum strain would improve the 3-methyl-1-butanol titer to 0.497 g/l after 12 h of incubation. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 关键词[WOS] | pyruvate-dehydrogenase complex ; escherichia-coli ; amino-acids ; decarboxylases ; fermentation ; mutagenesis ; alcohols ; enzyme ; gene |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374286500001 |
| 源URL | [http://ir.giec.ac.cn/handle/344007/11237]  |
| 专题 | 中国科学院广州能源研究所 |
| 作者单位 | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,et al. 3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum[J]. molecular biotechnology,2016,58(5):311-318. |
| APA | Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,Li, Xiekun,Zhang, Yu,&Yuan, Zhenhong.(2016).3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum.molecular biotechnology,58(5),311-318. |
| MLA | Xiao, Shiyuan,et al."3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum".molecular biotechnology 58.5(2016):311-318. |
入库方式： OAI收割
来源：广州能源研究所
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