中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Elucidating the Beneficial Effect of Corncob Acid Hydrolysate Environment on Lipid Fermentation of Trichosporon dermatis by Method of Cell Biology

文献类型:期刊论文

作者Huang, Chao1,2; Wang, Can1,2; Xiong, Lian1,2; Chen, Xue-Fang1,2; Lin, Xiao-Qing1,2; Qi, Gao-Xiang2,3; Shi, Si-Lan2,3; Wang, Bo2,3; Chen, Xin-De1,2,4
刊名applied biochemistry and biotechnology
出版日期2016-04-01
卷号178期号:7页码:1420-1429
关键词Corncob acid hydrolysate Trichosporon dermatis Flow cytometry Microbial oil Cell biology
英文摘要in present study, the beneficial effect of corncob acid hydrolysate environment on lipid fermentation of trichosporon dermatis was elucidated by method of cell biology (mainly using flow cytometry and microscope) for the first time. propidium iodide (pi) and rhodamine 123 (rh123) staining showed that corncob acid hydrolysate environment was favorable for the cell membrane integrity and mitochondrial membrane potential of t. dermatis and thus made its lipid fermentation more efficient. nile red (nr) staining showed that corncob acid hydrolysate environment made the lipid accumulation of t. dermatis slower, but this influence was not serious. moreover, the cell morphology of t. dermatis elongated in the corncob acid hydrolysate, but the cell morphology changed as elliptical-like during fermentation. overall, this work offers one simple and effective method to evaluate the influence of lignocellulosic hydrolysates environment on lipid fermentation.
WOS标题词science & technology ; life sciences & biomedicine
类目[WOS]biochemistry & molecular biology ; biotechnology & applied microbiology
研究领域[WOS]biochemistry & molecular biology ; biotechnology & applied microbiology
关键词[WOS]nile red fluorescence ; flow-cytometry ; oil production ; cider fermentation ; escherichia-coli ; yeast ; apoptosis ; accumulation ; mechanisms ; inhibitors
收录类别SCI
语种英语
WOS记录号WOS:000375614500012
源URL[http://ir.giec.ac.cn/handle/344007/11385]  
专题中国科学院广州能源研究所
作者单位1.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Huang, Chao,Wang, Can,Xiong, Lian,et al. Elucidating the Beneficial Effect of Corncob Acid Hydrolysate Environment on Lipid Fermentation of Trichosporon dermatis by Method of Cell Biology[J]. applied biochemistry and biotechnology,2016,178(7):1420-1429.
APA Huang, Chao.,Wang, Can.,Xiong, Lian.,Chen, Xue-Fang.,Lin, Xiao-Qing.,...&Chen, Xin-De.(2016).Elucidating the Beneficial Effect of Corncob Acid Hydrolysate Environment on Lipid Fermentation of Trichosporon dermatis by Method of Cell Biology.applied biochemistry and biotechnology,178(7),1420-1429.
MLA Huang, Chao,et al."Elucidating the Beneficial Effect of Corncob Acid Hydrolysate Environment on Lipid Fermentation of Trichosporon dermatis by Method of Cell Biology".applied biochemistry and biotechnology 178.7(2016):1420-1429.

入库方式: OAI收割

来源:广州能源研究所

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