Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate
文献类型：期刊论文
| 作者 | Liang Jiayuan; Sun Aijun; Zhang Yun; Deng Dun; Wang Yongfei; Ma Sanmei; Hu Yunfeng |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 34期号:6页码:1269-1277 |
| 关键词 | alkaline esterase kinetic resolution (R)-methyl mandelate high optical purity opposite stereoselectivity |
| 通讯作者 | Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China. ; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China. ; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China. |
| 中文摘要 | Genomic mining has identified a novel microbial alkaline esterase from the Indian Ocean. This esterase was overexpressed in E. coli BL21 (DE3) and further functionally characterized. Under optimal conditions (10 mmol/L substrate, pH 6.0, 2 h at 40 A degrees C), this esterase can hydrolyze racemic methyl mandelate to (R)-methyl mandelate with very high optical purity (e.e. > 99%) and yield (nearly 90%). Interestingly, the stereoselectivity of this esterase is opposite to that of two previously reported lipases that can generate (S)-methyl mandelate through the hydrolysis of racemic methyl mandelate. No organic solvents or other additives were required to optimize the optical purity and production of the final chiral product (R)-methyl mandelate, which can potentially simplify the production procedure of (R)-methyl mandelate catalyzed by esterase. |
| 学科主题 | Marine & Freshwater Biology; Oceanography |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15336]  |
| 专题 | 南海海洋研究所_广东省应用海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Liang Jiayuan,Sun Aijun,Zhang Yun,et al. Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2016,34(6):1269-1277. |
| APA | Liang Jiayuan.,Sun Aijun.,Zhang Yun.,Deng Dun.,Wang Yongfei.,...&Hu Yunfeng.(2016).Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(6),1269-1277. |
| MLA | Liang Jiayuan,et al."Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 34.6(2016):1269-1277. |
入库方式： OAI收割
来源：南海海洋研究所
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