Isolation and Characterization of a Glycosyl Hydrolase Family 16 beta-Agarase from a Mangrove Soil Metagenomic Library
文献类型：期刊论文
| 作者 | Mai, Zhimao; Su, Hongfei; Zhang, Si |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2016 |
| 卷号 | 17期号:8页码:1360 |
| 关键词 | agarase metagenomic library agar neoagaro-oligosaccharides |
| 通讯作者 | Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China. |
| 中文摘要 | A mangrove soil metagenomic library was constructed and a beta-agarase gene designated as AgaML was isolated by functional screening. The gene encoded for a 659-amino-acids polypeptide with an estimated molecular mass of 71.6 kDa. The deduced polypeptide sequences of AgaML showed the highest identity of 73% with the glycoside hydrolase family 16 beta-agarase from Microbulbifer agarilyticus in the GenBank database. AgaML was cloned and highly expressed in Escherichia coli BL21(DE3). The purified recombinant protein, AgaML, showed optimal activity at 50 degrees C and pH 7.0. The kinetic parameters of K-m and V-max values toward agarose were 4.6 mg.mL(-1) and 967.5 mu M.min(-1).mg(-1), respectively. AgaML hydrolyzed the beta-1,4-glycosidic linkages of agar to generate neoagarotetraose (NA4) and neoagarohexaose (NA6) as the main products. These characteristics suggest that AgaML has potential application in cosmetic, pharmaceuticals and food industries. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15337]  |
| 专题 | 南海海洋研究所_广东省应用海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Mai, Zhimao,Su, Hongfei,Zhang, Si. Isolation and Characterization of a Glycosyl Hydrolase Family 16 beta-Agarase from a Mangrove Soil Metagenomic Library[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(8):1360. |
| APA | Mai, Zhimao,Su, Hongfei,&Zhang, Si.(2016).Isolation and Characterization of a Glycosyl Hydrolase Family 16 beta-Agarase from a Mangrove Soil Metagenomic Library.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(8),1360. |
| MLA | Mai, Zhimao,et al."Isolation and Characterization of a Glycosyl Hydrolase Family 16 beta-Agarase from a Mangrove Soil Metagenomic Library".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.8(2016):1360. |
入库方式： OAI收割
来源：南海海洋研究所
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