Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas
文献类型：期刊论文
| 作者 | Xu, Fengjiao; Zhang, Yang; Li, Jun; Zhang, Yuehuan; Xiang, Zhiming; Yu, Ziniu |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 45期号:2页码:510-516 |
| 关键词 | Crassostrea gigas Truncated Myd88 variants Rel/NF-kappa B TIR domain |
| 通讯作者 | yzhang@scsio.ac.cn |
| 中文摘要 | Myeloid differentiation factor 88 (MyD88) is the classic signaling adaptor that mediates Toll/interleukin-1 receptor (TIR/IL-1R) dependent activation of nuclear factor-kappa B (NF-kappa B). In this study, two naturally truncated MyD88 members were identified from the Pacific oyster (Crassostrea gigas), namely CgMyD88-T1 and CgMyD88-T2. The full-length cDNA of CgMyD88-T1, CgMyD88-T2 are 976 bp and 1038 bp in length, containing an ORF of 552 bp and 555 bp, respectively. The two ORF encode a putative protein of 183 and 184 amino acids, respectively, with a calculated molecular weight of about 21 and 22 kDa. When compared to complete MyD88 paralogues, we found that both CgMyD88-T1 and CgMyD88-T2 contain only TIR domain but lack DD (Death Domain), which share 90.8% of similarity and 71.7% of identity with each other. Phylogenetic tree demonstrated that CgMyD88-T1 and CgMyD88-T2 clustered together and belonged to mollusk branch. Meanwhile, genomic arrangement analysis displayed that the two truncated MyD88s were distributed in tandem in one scaffold, revealing that they may originate from one truncated MyD88 ancestor recently. Expression profile showed that both of CgMyD88 variants were ubiquitously expressed in all tested tissues with highest expression in the gills and hemocytes, respectively. Both truncated CgMyD88 mRNAs were significantly up-regulated in hemocytes under HKLM (heat-killed Listeria monocytogenes) and HKVA (heat-killed Vibrio alginolyticus) challenge. Moreover, either CgMyD88-T1 or CgMyD88-T2 were able to inhibit MyD88 activated Rel/NF-kappa B activity in HEK293 cell, demonstrating their negative role in regulating MyD88-mediated immune signaling. (C) 2015 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 收录类别 | SCI |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15054]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Xu, Fengjiao,Zhang, Yang,Li, Jun,et al. Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):510-516. |
| APA | Xu, Fengjiao,Zhang, Yang,Li, Jun,Zhang, Yuehuan,Xiang, Zhiming,&Yu, Ziniu.(2015).Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,45(2),510-516. |
| MLA | Xu, Fengjiao,et al."Expression and function analysis of two naturally truncated MyD88 variants in the Pacific oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):510-516. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。