Foxl2 of the Hong Kong catfish (Clarias fuscus): cDNA cloning, tissue distribution and changes in gene expression towards methyltestosterone, estradiol and letrozole exposure of the fries during gonadal differentiation
文献类型：期刊论文
| 作者 | Deng, Si-ping; Zhu, Chun-hua; Sun, Jing; Wang, Wen-da; Wu, Tian-li; Chen, Hua-pu; Shi, Shang-li; Li, Guang-li |
| 刊名 | GENES & GENOMICS
 |
| 出版日期 | 2015 |
| 卷号 | 37期号:8页码:669-677 |
| 关键词 | Methyltestosterone Estradiol Letrozole Clarias fuscus foxl2 |
| 通讯作者 | guangli211@163.com |
| 中文摘要 | Winged helix/forkhead transcription factor gene 2 (Foxl2) plays a crucial role during early ovarian development in fish. Sex steroids and aromatase inhibitors can regulate gonadal differentiation in teleosts. To address the role of Foxl2 in gonadal differentiation, foxl2 was isolated and characterized from the Hong Kong catfish, Clarias fuscus. Tissue distribution analysis by quantitative real-time PCR (qPCR) showed that the foxl2 mRNA was highest in the ovaries; moderate in the female pituitary and hypothalamus; but weak in the forebrain, liver, testis and male pituitary, with a sexually dimorphic pattern in which there was higher expression overall in females versus males. Treatment of the fries during the period of gonadal differentiation [2-30 days post hatching (dph)] with 17 alpha-methyltestosterone (MT), an aromatase inhibitor (letrozole) and 17 beta-estradiol (E-2) affected the expression of foxl2. The expression of foxl2 was highest before gonadal differentiation (12 dph), but decreased significantly after gonadal differentiation (18-30 dph). In addition, MT and letrozole were able to down-regulate foxl2, but E-2 up-regulated foxl2 during gonadal morphological differentiation. These results indicate the significant roles of Foxl2 in the gonadal differentiation of C. fuscus. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity |
| 收录类别 | SCI |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15062]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Deng, Si-ping,Zhu, Chun-hua,Sun, Jing,et al. Foxl2 of the Hong Kong catfish (Clarias fuscus): cDNA cloning, tissue distribution and changes in gene expression towards methyltestosterone, estradiol and letrozole exposure of the fries during gonadal differentiation[J]. GENES & GENOMICS,2015,37(8):669-677. |
| APA | Deng, Si-ping.,Zhu, Chun-hua.,Sun, Jing.,Wang, Wen-da.,Wu, Tian-li.,...&Li, Guang-li.(2015).Foxl2 of the Hong Kong catfish (Clarias fuscus): cDNA cloning, tissue distribution and changes in gene expression towards methyltestosterone, estradiol and letrozole exposure of the fries during gonadal differentiation.GENES & GENOMICS,37(8),669-677. |
| MLA | Deng, Si-ping,et al."Foxl2 of the Hong Kong catfish (Clarias fuscus): cDNA cloning, tissue distribution and changes in gene expression towards methyltestosterone, estradiol and letrozole exposure of the fries during gonadal differentiation".GENES & GENOMICS 37.8(2015):669-677. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。