Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities
文献类型：期刊论文
| 作者 | Wan Peng; Yang Xiaoman; Cai Bingna; Chen Hua; Sun Huili; Chen Deke; Pan Jianyu |
| 刊名 | JOURNAL OF OCEAN UNIVERSITY OF CHINA
 |
| 出版日期 | 2015 |
| 卷号 | 14期号:4页码:651-662 |
| 关键词 | polysaccharides Laminaria japonica ultrasonic extraction antioxidative alpha-glucosidase alpha-amylase |
| 通讯作者 | jypan@scsio.ac.cn |
| 中文摘要 | In the present study, ultrasonic extraction technique (UET) is used to improve the yield of polysaccharides from Laminaria japonica (LJPs). And their antioxidative as well as glycosidase inhibitory activities are investigated. Box-Behnken design (BBD) combined with response surface methodology (RSM) is applied to optimize ultrasonic extraction for polysaccharides. The optimized conditions are obtained as extraction time at 54 min, ultrasonic power at 1050 W, extraction temperature at 80A degrees C and ratio of material to solvent at 1:50 (g mL(-1)). Under these optimal ultrasonic extraction conditions, an actual experimental yield (5.75% +/- 0.3%) is close to the predicted result (5.67%) with no significant difference (P > 0.05). Vitro antioxidative and glycosidase inhibitory activities tests indicate that the crude polysaccharides (LJP) and two major ethanol precipitated fractions (LJP1 and LJP2) are in a concentration-dependent manner. LJP2 (30%-60% ethanol precipitated polysaccharides) possesses the strongest alpha-glucosidase inhibitory activity and moderate scavenging activity against hydroxyl radicals (66.09% +/- 2.19%, 3.0 mg mL(-1)). Also, the inhibitory activity against alpha-glucosidase (59.08% +/- 3.79%, 5.0 mg mL(-1)) is close to that of acarbose (63.99% +/- 3.27%, 5.0 mg mL(-1)). LJP1 (30% ethanol precipitated polysaccharides) exhibits the strongest scavenging activity against hydroxyl radicals (99.80% +/- 0.00%, 3.0 mg mL(-1)) and moderate alpha-glucosidase inhibitory activity (47.76% +/- 1.92%, 5.0 mg mL(-1)). LJP shows the most remarkable DPPH scavenging activity (66.20% +/- 0.11%, 5.0 mg mL(-1)) but weakest alpha-glucosidase inhibitory activity (37.77% +/- 1.30%, 5.0 mg mL(-1)). However, all these LJPs exert weak inhibitory effects against alpha-amylase. These results show that UET is an effective method for extracting bioactive polysaccharides from seaweed materials. LJP1 and LJP2 can be developed as a potential ingredient in hypoglycemic agents or functional food for the management of diabetes. This study provides scientific evidence and advances in the preparation technology and a hypoglycemic activities evaluation method for seaweed polysaccharides, especially glycosidase inhibition in combination with an antioxidative activity evaluation method. |
| 学科主题 | Oceanography |
| 收录类别 | SCI |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15064]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Wan Peng,Yang Xiaoman,Cai Bingna,et al. Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2015,14(4):651-662. |
| APA | Wan Peng.,Yang Xiaoman.,Cai Bingna.,Chen Hua.,Sun Huili.,...&Pan Jianyu.(2015).Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities.JOURNAL OF OCEAN UNIVERSITY OF CHINA,14(4),651-662. |
| MLA | Wan Peng,et al."Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities".JOURNAL OF OCEAN UNIVERSITY OF CHINA 14.4(2015):651-662. |
入库方式： OAI收割
来源：南海海洋研究所
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