Comprehensive identification and profiling of host miRNAs in response to Singapore grouper iridovirus (SGIV) infection in grouper (Epinephelus coioides)
文献类型：期刊论文
| 作者 | Guo, Chuanyu; Cui, Huachun; Ni, Songwei; Yan, Yang; Qin, Qiwei |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 52期号:2页码:226-235 |
| 关键词 | microRNAs Grouper SGIV Deep sequencing miRNA microarray Functional annotation |
| 通讯作者 | yanyang@scsio.ac.cn ; qinqw@scsio.ac.cn |
| 中文摘要 | microRNAs (miRNAs) are an evolutionarily conserved class of non-coding RNA molecules that participate in various biological processes. Employment of high-throughput screening strategies greatly prompts the investigation and profiling of miRNAs in diverse species. In recent years, grouper (Epinephelus spp.) aquaculture was severely affected by iridoviral diseases. However, knowledge regarding the host immune responses to viral infection, especially the miRNA-mediated immune regulatory roles, is rather limited. In this study, by employing Solexa deep sequencing approach, we identified 116 grouper miRNAs from grouper spleen-derived cells (GS). As expected, these miRNAs shared high sequence similarity with miRNAs identified in zebrafish (Danio rerio), pufferfish (Fugu rubripes), and other higher vertebrates. In the process of Singapore grouper iridovirus (SGIV) infection, 45 and 43 miRNAs with altered expression (>1.5-fold) were identified by miRNA microarray assays in grouper spleen tissues and GS cells, respectively. Furthermore, target prediction revealed 189 putative targets of these grouper miRNAs. (C) 2015 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology; Zoology |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14914]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Guo, Chuanyu,Cui, Huachun,Ni, Songwei,et al. Comprehensive identification and profiling of host miRNAs in response to Singapore grouper iridovirus (SGIV) infection in grouper (Epinephelus coioides)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,52(2):226-235. |
| APA | Guo, Chuanyu,Cui, Huachun,Ni, Songwei,Yan, Yang,&Qin, Qiwei.(2015).Comprehensive identification and profiling of host miRNAs in response to Singapore grouper iridovirus (SGIV) infection in grouper (Epinephelus coioides).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,52(2),226-235. |
| MLA | Guo, Chuanyu,et al."Comprehensive identification and profiling of host miRNAs in response to Singapore grouper iridovirus (SGIV) infection in grouper (Epinephelus coioides)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 52.2(2015):226-235. |
入库方式： OAI收割
来源：南海海洋研究所
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