A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action
文献类型：期刊论文
| 作者 | Zhang, Linbao; Yang, Dinglong; Wang, Qing; Yuan, Zeyi; Wu, Huifeng; Pei, Dong; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 51期号:1页码:29-38 |
| 关键词 | Venerupis philippinarum Defensin Molecular characterization Tissue distribution Antimicrobial activity Membrane lesions |
| 通讯作者 | hfwu@yic.ac.cn ; jmzhao@yic.ac.cn |
| 中文摘要 | Antimicrobial peptides (AMPs) are important mediators of the primary host defense system against microbial invasion. In the present study, we cloned and characterized a member of the invertebrate defensin from the clam Venerupis philippinarum, designated VpDef. Amino acid sequence analysis showed that VpDef was similar to defensins from marine mollusks and ticks. In non-stimulated clams, RT-PCR and immunohistochemical analysis revealed that both VpDef mRNA and the encoding peptide were constitutively expressed in hemocytes and mantles, as well as in other major tissues. VpDef transcripts were significantly induced in hemocytes at different time intervals post Vibrio anguillarum infection. The recombinant VpDef (rVpDef) showed the highest activity against Gram-positive bacteria Micrococcus luteus and less effective to Gram-negative bacteria. In addition, incubation of rVpDef with M. luteus at 1 x and 3 x MIC could induce an obvious decrease of the membrane potential and notable changes of membrane permeability in a dose-dependent manner. Membrane integrity and bacterial viability analysis also revealed that rVpDef increased the membrane permeability of M. luteus and then resulted in cell death at 2 x and 10 x MIC. Overall, these results suggest that VpDef has an important function in host defense against invasive pathogens, probably killing microbes by inducing membrane lesions. (C) 2015 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology; Zoology |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14963]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Zhang, Linbao,Yang, Dinglong,Wang, Qing,et al. A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):29-38. |
| APA | Zhang, Linbao.,Yang, Dinglong.,Wang, Qing.,Yuan, Zeyi.,Wu, Huifeng.,...&Zhao, Jianmin.(2015).A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),29-38. |
| MLA | Zhang, Linbao,et al."A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):29-38. |
入库方式： OAI收割
来源：南海海洋研究所
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