A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis
文献类型：期刊论文
| 作者 | Qu, Fufa; Xiang, Zhiming; Wang, Fuxuan; Zhang, Yang; Tong, Ying; Li, Jun; Zhang, Yuehuan; Yu, Ziniu |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 51期号:1页码:194-201 |
| 关键词 | C. hongkongensis Innate immunity AP-1 pathway Fos |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 中文摘要 | The transcription factor Fos is a member of one of the best-studied AP-1 sub-families and has been implicated in a wide variety of biological processes, including the regulation of apoptosis, immune responses and cytokine production. In this report, a novel mollusk Fos (referred to as ChFos) gene was cloned and characterized from the Hong Kong oyster, Crassostrea hongkongensis. The deduced ChFos protein sequence comprised 333 amino acids and shared significant homology with invertebrate homologs. Phylogenetic analysis revealed that ChFos clusters with Fos from Crassostrea gigas and Crassostrea ariakensis. Quantitative real-time PCR analysis revealed that ChFos mRNA was broadly expressed in all tested tissues and during different stages of the oyster's embryonic and larval development. In addition, the expression of ChFos mRNA was significantly up-regulated under challenge with microorganisms (Vibrio alginolyticus, Staphylococcus haemolyticus and Saccharomyces cerevisiae) and pathogen-associated molecular patterns (PAMPs: LPS, PGN and polyl:C). Moreover, fluorescence microscopy showed that ChFos protein is localized in the nucleus in HEK293T cells. Reporter assays suggested that ChFos may act as an efficient transcription activator in the regulation of AP-1-responsive gene expression through interaction with ChJun. Overall, this study presents the first experimental evidence of the presence and functional characteristics of Fos in mollusks, which reveals its involvement in host protection against immune challenge in the oyster. (C) 2015 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology; Zoology |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14964]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | Qu, Fufa,Xiang, Zhiming,Wang, Fuxuan,et al. A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):194-201. |
| APA | Qu, Fufa.,Xiang, Zhiming.,Wang, Fuxuan.,Zhang, Yang.,Tong, Ying.,...&Yu, Ziniu.(2015).A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),194-201. |
| MLA | Qu, Fufa,et al."A novel molluscan Fos gene with immune defense function identified in the Hong Kong oyster, Crassostrea hongkongensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):194-201. |
入库方式： OAI收割
来源：南海海洋研究所
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