Rh protein expression in branchial neuroepithelial cells, and the role of ammonia in ventilatory control in fish
文献类型：期刊论文
| 作者 | Zhang, Li1; Nawata, C. Michele1; De Boeck, Gudrun1; Wood, Chris M.1 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 186页码:39-51 |
| 关键词 | Ammonia Teleost fish Elasmobranchs Chemoreceptors Rhesus glycoproteins Neuroepithelial cells Ventilation Brain 1st gill arch Serotonin |
| 通讯作者 | woodcm@zoology.ubc.ca |
| 英文摘要 | Bill Milsom has made seminal contributions to our understanding of ventilatory control in a wide range of vertebrates. Teleosts are particularly interesting, because they produce a 3rd, potentially toxic respiratory gas (ammonia) in large amounts. Fish are well known to hyperventilate under high environmental ammonia (HEA), but only recently has the potential role of ammonia in normal ventilatory control been investigated. It is now clear that ammonia can act directly as a ventilatory stimulant in trout, independent of its effects on acid-base balance. Even in ureotelic dogfish sharks, acute elevations in ammonia cause increases in ventilation. Peripherally, the detection of elevated ammonia resides in gill arches I and II in trout, and in vitro, neuroepithelial cells (NECs) from these arches are sensitive to ammonia, responding with elevations in intracellular Ca2+ ([Ca2+](i)). Centrally, hyperventilatory responses to ammonia correlate more closely with concentrations of ammonia in the brain than in plasma or CSF. After chronic HEA exposure, ventilatory responsiveness to ammonia is lost, associated with both an attenuation of the [Ca2+](i); response in NECs, and the absence of elevation in brain ammonia concentration. Chronic exposure to HEA also causes increases in the mRNA expression of several Rh proteins (ammonia-conductive channels) in both brain and gills. "Single cell" PCR techniques have been used to isolate the individual responses of NECs versus other gill cell types. We suggest several circumstances (post-feeding, post-exercise) where the role of ammonia as a ventilatory stimulant may have adaptive benefits for O-2 uptake in fish. (C) 2014 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Physiology; Zoology |
| 原文出处 | 1095-6433 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14796]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Zhang, Li 2.Nawata, C. Michele 3.Wood, Chris M.] McMaster Univ, Dept Biol, Hamilton, ON, Canada 4.[Zhang, Li] South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou, Guangdong, Peoples R China 5.[Nawata, C. Michele] Univ Arizona, Dept Physiol, Tucson, AZ USA 6.[Nawata, C. Michele 7.De Boeck, Gudrun 8.Wood, Chris M.] Bamfield Marine Sci Ctr, Bamfield, BC, Canada 9.[De Boeck, Gudrun] Univ Antwerp, Dept Biol, SPHERE, B-2020 Antwerp, Belgium 10.[Wood, Chris M.] Univ British Columbia, Dept Zool, Vancouver, BC V6T 1Z4, Canada |
| 推荐引用方式 GB/T 7714 | Zhang, Li,Nawata, C. Michele,De Boeck, Gudrun,et al. Rh protein expression in branchial neuroepithelial cells, and the role of ammonia in ventilatory control in fish[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2015,186:39-51. |
| APA | Zhang, Li,Nawata, C. Michele,De Boeck, Gudrun,&Wood, Chris M..(2015).Rh protein expression in branchial neuroepithelial cells, and the role of ammonia in ventilatory control in fish.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,186,39-51. |
| MLA | Zhang, Li,et al."Rh protein expression in branchial neuroepithelial cells, and the role of ammonia in ventilatory control in fish".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 186(2015):39-51. |
入库方式： OAI收割
来源：南海海洋研究所
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