Cloning and characterization of three suppressors of cytokine signaling (SOCS) genes from the Pacific oyster, Crassostrea gigas
文献类型：期刊论文
| 作者 | Li, Jun1; Zhang, Yang1; Zhang, Yuehuan1; Liu, Ying1; Xiang, Zhiming1; Qu, Fufa1; Yu, Ziniu1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 44期号:2页码:525-532 |
| 关键词 | Suppressor of cytokine signaling PAMP challenge Crassostrea gigas |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 英文摘要 | Members of the suppressor of cytokine signaling (SOCS) family are crucial for the control of a variety of signal transduction pathways that are involved in the immunity, growth and development of organisms. However, in mollusks, the identity and function of SOCS proteins remain largely unclear. In the present study, three SOCS genes, CgSOCS2, CgSOCS5 and CgSOCS7, have been identified by searching and analyzing the Pacific oyster genome. Structural analysis indicated that the CgSOCS share conserved functional domains with their vertebrate counterparts. Phylogenetic analysis showed that the three SOCS genes clustered into two distinct groups, the type I and II subfamilies, indicating that these subfamilies had common ancestors. Tissue-specific expression results showed that the three genes were constitutively expressed in all examined tissues and were highly expressed in immune-related tissues, such as the hemocytes, gills and digestive gland. The expression of CgSOCS can also be induced to varying degrees in hemocytes after challenge with pathogen-associated molecular patterns (PAMPs). Moreover, dual-luciferase reporter assays showed that the over-expression of CgSOCS2 and CgSOCS7, but not CgSOC5, can activate an NF-kappa B reporter gene. Collectively, these results demonstrated that the CgSOCS might play an important role in the innate immune responses of the Pacific oyster. (c) 2015 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 原文出处 | 1050-4648 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14826]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Li, Jun 2.Zhang, Yang 3.Zhang, Yuehuan 4.Liu, Ying 5.Xiang, Zhiming 6.Qu, Fufa 7.Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 8.[Liu, Ying 9.Qu, Fufa] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jun,Zhang, Yang,Zhang, Yuehuan,et al. Cloning and characterization of three suppressors of cytokine signaling (SOCS) genes from the Pacific oyster, Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2015,44(2):525-532. |
| APA | Li, Jun.,Zhang, Yang.,Zhang, Yuehuan.,Liu, Ying.,Xiang, Zhiming.,...&Yu, Ziniu.(2015).Cloning and characterization of three suppressors of cytokine signaling (SOCS) genes from the Pacific oyster, Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,44(2),525-532. |
| MLA | Li, Jun,et al."Cloning and characterization of three suppressors of cytokine signaling (SOCS) genes from the Pacific oyster, Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 44.2(2015):525-532. |
入库方式： OAI收割
来源：南海海洋研究所
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