Role of two amino acid residues' insertion on thermal stability of thermophilic alpha-amylase AMY121 from a deep sea bacterium Bacillus sp SCSIO 15121
文献类型：期刊论文
| 作者 | Li, Lizhen1; Yang, Jian1; Li, Jie1; Long, Lijuan1; Xiao, Yunzhu1; Tian, Xinpeng1; Wang, Fazuo1; Zhang, Si1 |
| 刊名 | BIOPROCESS AND BIOSYSTEMS ENGINEERING
 |
| 出版日期 | 2015 |
| 卷号 | 38期号:5页码:871-879 |
| 关键词 | alpha-Amylases Thermal stability Site-directed mutagenesis Calcium-binding sites Deep sea bacterium |
| 通讯作者 | yangjian@scsio.ac.cn ; zhsimd@scsio.ac.cn |
| 英文摘要 | alpha-Amylases from Bacillus licheniformis (BLA) and Bacillus amyloliquefaciens (BAA) are both important industrial enzymes with high similarity in structure but significant differences in thermostability. The mechanisms underlying this discrepancy are still poorly understood. Here, we investigated the role of two amino acids' insertion on the thermostability of these two group amylases. A newly obtained thermophilic amylase AMY121 was found much closer to BLA in both primary structure and enzymological properties. Two amino acids' insertion widespread among BAA group alpha-amylases was identified as one of the key factors leading to the thermostability differences, since thermostability of insertion mutants (AMY121-EG and AMY121-AA) from AMY121 significantly decreased, while that of deletion mutant from BAA increased. Moreover, we proposed that conformational disturbance caused by insertion mutation might weaken the calcium-binding affinity and consequently decrease the enzyme thermostability. |
| 学科主题 | Biotechnology & Applied Microbiology; Engineering |
| 原文出处 | 1615-7591 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14839]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Li, Lizhen 2.Yang, Jian 3.Li, Jie 4.Long, Lijuan 5.Xiao, Yunzhu 6.Tian, Xinpeng 7.Wang, Fazuo 8.Zhang, Si] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China 9.[Li, Lizhen 10.Xiao, Yunzhu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Lizhen,Yang, Jian,Li, Jie,et al. Role of two amino acid residues' insertion on thermal stability of thermophilic alpha-amylase AMY121 from a deep sea bacterium Bacillus sp SCSIO 15121[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2015,38(5):871-879. |
| APA | Li, Lizhen.,Yang, Jian.,Li, Jie.,Long, Lijuan.,Xiao, Yunzhu.,...&Zhang, Si.(2015).Role of two amino acid residues' insertion on thermal stability of thermophilic alpha-amylase AMY121 from a deep sea bacterium Bacillus sp SCSIO 15121.BIOPROCESS AND BIOSYSTEMS ENGINEERING,38(5),871-879. |
| MLA | Li, Lizhen,et al."Role of two amino acid residues' insertion on thermal stability of thermophilic alpha-amylase AMY121 from a deep sea bacterium Bacillus sp SCSIO 15121".BIOPROCESS AND BIOSYSTEMS ENGINEERING 38.5(2015):871-879. |
入库方式： OAI收割
来源：南海海洋研究所
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