Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper
文献类型：期刊论文
| 作者 | Wei, Jingguang1; Gao, Pin1; Zhang, Ping1; Guo, Minglan1; Xu, Meng1; Wei, Shina1; Yan, Yang1; Qin, Qiwei1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 43期号:2页码:396-404 |
| 关键词 | Epinephelus coioides Apolipoprotein A-I Innate immunity Intracellular localization |
| 通讯作者 | qinqw@scsio.ac.cn |
| 英文摘要 | Apolipoproteins, synthesized mainly in liver and intestine and bounded to lipids, play important roles in lipid transport and uptake through the circulation system. In this study, an apolipoprotein A-I gene homologue was cloned from orange-spotted grouper Epinephelus coioides (designed as Ec-ApoA-I) by rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of Ec-ApoA-I was comprised of 1278 bp with a 792 bp open reading frame (ORF) that encodes a putative protein of 264 amino acids. Quantitative real-time PCR (qPCR) analysis revealed that Ec-ApoA-I was abundant in liver and intestine, and the expression in liver was significantly (P < 0.01) up-regulated after the stimulation of LPS, Poly(I:C), Vibrio alginolyticus, and Singapore grouper iridovirus (SGIV). Recombinant Ec-ApoA-I (rEc-ApoA-I) was produced in Escherichia coli BL21 (DE3) expression system exhibited bacteriolyticactivity against Microcococcus lysodeikticus and Aeromonas hydrophila. Intracellular localization revealed that Ec-ApoA-I distributed in both cytoplasm and nucleus, and predominantly in the cytoplasm. Overexpression of Ec-ApoA-I in grouper Brain (GB) cells could inhibit the replication of SGIV. These results together indicated that Ec-ApoA-I perhaps is involved in the responses to bacterial and viral challenge. (C) 2015 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 原文出处 | 1050-4648 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14846]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Wei, Jingguang 2.Guo, Minglan 3.Wei, Shina 4.Yan, Yang 5.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 6.[Wei, Jingguang 7.Guo, Minglan 8.Wei, Shina 9.Yan, Yang 10.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Jingguang,Gao, Pin,Zhang, Ping,et al. Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper[J]. FISH & SHELLFISH IMMUNOLOGY,2015,43(2):396-404. |
| APA | Wei, Jingguang.,Gao, Pin.,Zhang, Ping.,Guo, Minglan.,Xu, Meng.,...&Qin, Qiwei.(2015).Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper.FISH & SHELLFISH IMMUNOLOGY,43(2),396-404. |
| MLA | Wei, Jingguang,et al."Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper".FISH & SHELLFISH IMMUNOLOGY 43.2(2015):396-404. |
入库方式： OAI收割
来源：南海海洋研究所
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