Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China
文献类型：期刊论文
| 作者 | Huang, X.1; Huang, Y.1; Xu, L.1; Wei, S.1; Ouyang, Z.1; Feng, J.1; Qin, Q.1 |
| 刊名 | JOURNAL OF FISH DISEASES
 |
| 出版日期 | 2015 |
| 卷号 | 38期号:4页码:379-387 |
| 关键词 | DNA polymerase grouper iridovirus lymphocystis disease virus major capsid protein |
| 通讯作者 | qinqw@scsio.ac.cn |
| 英文摘要 | Grouper Epinephelus spp. is one of the most important mariculture fish species in China and South-East Asian countries. The emerging viral diseases, evoked by iridovirus which belongs to genus Megalocytivirus and Ranavirus, have been well characterized in recent years. To date, few data on lymphocystis disease in grouper which caused by lymphocystis disease virus (LCDV) were described. Here, a novel LCDV isolate was identified and characterized. Based on the sequence of LCDV major capsid protein (MCP) and DNA polymerase gene, we found that the causative agents from different species of diseased groupers were the same one and herein were uniformly defined as grouper LCDV (GLCDV). Furthermore, H& E staining revealed that the nodules on the skin were composed of giant cells that contained inclusion bodies in the cytoplasm. Numerous virus particles with >210 nm in diameter and with hexagonal profiles were observed in the cytoplasm. In addition, phylogenetic analysis based on four iridovirus core genes, MCP, DNA polymerase, myristoylated membrane protein (MMP) and ribonucleotide reductase (RNR), consistently showed that GLCDV was mostly related to LCDV-C, followed by LCDV-1. Taken together, our data firstly provided the molecular evidence that GLCDV was a novel emerging iridovirus pathogen in grouper culture. |
| 学科主题 | Fisheries; Marine & Freshwater Biology; Veterinary Sciences |
| 原文出处 | 0140-7775 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14849]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Huang, X. 2.Huang, Y. 3.Wei, S. 4.Ouyang, Z. 5.Qin, Q.] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 6.[Xu, L. 7.Feng, J.] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, X.,Huang, Y.,Xu, L.,et al. Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China[J]. JOURNAL OF FISH DISEASES,2015,38(4):379-387. |
| APA | Huang, X..,Huang, Y..,Xu, L..,Wei, S..,Ouyang, Z..,...&Qin, Q..(2015).Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China.JOURNAL OF FISH DISEASES,38(4),379-387. |
| MLA | Huang, X.,et al."Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China".JOURNAL OF FISH DISEASES 38.4(2015):379-387. |
入库方式： OAI收割
来源：南海海洋研究所
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