中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China

文献类型:期刊论文

作者Huang, X.1; Huang, Y.1; Xu, L.1; Wei, S.1; Ouyang, Z.1; Feng, J.1; Qin, Q.1
刊名JOURNAL OF FISH DISEASES
出版日期2015
卷号38期号:4页码:379-387
关键词DNA polymerase grouper iridovirus lymphocystis disease virus major capsid protein
通讯作者qinqw@scsio.ac.cn
英文摘要Grouper Epinephelus spp. is one of the most important mariculture fish species in China and South-East Asian countries. The emerging viral diseases, evoked by iridovirus which belongs to genus Megalocytivirus and Ranavirus, have been well characterized in recent years. To date, few data on lymphocystis disease in grouper which caused by lymphocystis disease virus (LCDV) were described. Here, a novel LCDV isolate was identified and characterized. Based on the sequence of LCDV major capsid protein (MCP) and DNA polymerase gene, we found that the causative agents from different species of diseased groupers were the same one and herein were uniformly defined as grouper LCDV (GLCDV). Furthermore, H& E staining revealed that the nodules on the skin were composed of giant cells that contained inclusion bodies in the cytoplasm. Numerous virus particles with >210 nm in diameter and with hexagonal profiles were observed in the cytoplasm. In addition, phylogenetic analysis based on four iridovirus core genes, MCP, DNA polymerase, myristoylated membrane protein (MMP) and ribonucleotide reductase (RNR), consistently showed that GLCDV was mostly related to LCDV-C, followed by LCDV-1. Taken together, our data firstly provided the molecular evidence that GLCDV was a novel emerging iridovirus pathogen in grouper culture.
学科主题Fisheries; Marine & Freshwater Biology; Veterinary Sciences
原文出处0140-7775
源URL[http://ir.scsio.ac.cn/handle/344004/14849]  
专题南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位1.[Huang, X.
2.Huang, Y.
3.Wei, S.
4.Ouyang, Z.
5.Qin, Q.] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
6.[Xu, L.
7.Feng, J.] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Huang, X.,Huang, Y.,Xu, L.,et al. Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China[J]. JOURNAL OF FISH DISEASES,2015,38(4):379-387.
APA Huang, X..,Huang, Y..,Xu, L..,Wei, S..,Ouyang, Z..,...&Qin, Q..(2015).Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China.JOURNAL OF FISH DISEASES,38(4),379-387.
MLA Huang, X.,et al."Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China".JOURNAL OF FISH DISEASES 38.4(2015):379-387.

入库方式: OAI收割

来源:南海海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。