Effect of acute ammonia exposure on expression of GH/IGF axis genes GHR1, GHR2 and IGF-1 in pufferfish (Takifugu obscurus)
文献类型：期刊论文
| 作者 | Cheng, Chang-Hong1; Yang, Fang-Fang1; Liao, Shao-An1; Miao, Yu-Tao1; Ye, Chao-Xia1; Wang, An-Li1 |
| 刊名 | FISH PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2015 |
| 卷号 | 41期号:2页码:495-507 |
| 关键词 | Pufferfish Environmental stress Cloning Real-time PCR |
| 通讯作者 | chengchanghong09@163.com ; chaoxia_ye@yahoo.com.cn ; wanganl@scnu.edu.cn |
| 英文摘要 | Waterborne ammonia has become a persistent pollutant of aquatic habitats. The exposure to ammonia stress can reduce growth in a wide range of aquatic organisms. To assess the effect of ammonia exposure on the growth hormone/insulin-like growth factors (GH/IGF) axis, we identified and characterized GHR1, GHR2 and IGF-1 from pufferfish. Comparative analysis showed that these genes shared high identity and similarity with corresponding genes in other fish species. The transcripts of these genes were widely expressed in all tested tissues. The highest level of GHR1 mRNA was found in the brain, whereas GHR2 and IGF-1 mRNA levels were the highest in the liver. Following acute ammonia exposure (100 mg/L total ammonia-nitrogen), GHR2 expression in the liver did not change at 6 h and then significantly decreased at 12, 24 and 48 h, whereas GHR1 and IGF-1 expressions were significantly down-regulated at 6, 12, 24 and 48 h, respectively. These results indicated that ammonia stress decreased the expression of GH/IGF axis genes, which might have negative effect on the growth and development of pufferfish. |
| 学科主题 | Biochemistry & Molecular Biology; Fisheries; Physiology |
| 原文出处 | 0920-1742 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14851]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Cheng, Chang-Hong 2.Liao, Shao-An 3.Miao, Yu-Tao 4.Ye, Chao-Xia 5.Wang, An-Li] S China Normal Univ, Sch Life Sci, Key Lab Ecol & Environm Sci, Guangdong Prov Key Lab Hlth & Safe Aquaculture,Gu, Guangzhou 510631, Guangdong, Peoples R China 6.[Yang, Fang-Fang] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Chang-Hong,Yang, Fang-Fang,Liao, Shao-An,et al. Effect of acute ammonia exposure on expression of GH/IGF axis genes GHR1, GHR2 and IGF-1 in pufferfish (Takifugu obscurus)[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(2):495-507. |
| APA | Cheng, Chang-Hong,Yang, Fang-Fang,Liao, Shao-An,Miao, Yu-Tao,Ye, Chao-Xia,&Wang, An-Li.(2015).Effect of acute ammonia exposure on expression of GH/IGF axis genes GHR1, GHR2 and IGF-1 in pufferfish (Takifugu obscurus).FISH PHYSIOLOGY AND BIOCHEMISTRY,41(2),495-507. |
| MLA | Cheng, Chang-Hong,et al."Effect of acute ammonia exposure on expression of GH/IGF axis genes GHR1, GHR2 and IGF-1 in pufferfish (Takifugu obscurus)".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.2(2015):495-507. |
入库方式： OAI收割
来源：南海海洋研究所
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