Isolation and characterization of a Klebsiella pneumoniae strain from mangrove sediment for efficient biosynthesis of 1,3-propanediol
文献类型：期刊论文
| 作者 | Zhou, Sheng1; Li, Lili1; Perseke, Marleen1; Huang, Youhua1; Wei, Jingguang1; Qin, Qiwei1 |
| 刊名 | SCIENCE BULLETIN
 |
| 出版日期 | 2015 |
| 卷号 | 60期号:5页码:511-521 |
| 关键词 | Marine Klebsiella pneumoniae Fermentation 1,3-Propanediol Metabolism |
| 通讯作者 | qinqw@scsio.ac.cn |
| 英文摘要 | 1,3-Propanediol (PDO) is an important bulk industrial material. It can be produced by microbial fermentation. In this study, the microbial flora of mangrove sediment was screened to identify strains with high production of PDO by fermentation of glycerol. The PDO productivities of the isolated strains were tested, and the strain with highest PDO productivity was characterized using the API20E and 16-s rRNA sequence analysis. The physiological and phylogenetic analysis indicated that the strain was closed related to K. pneumoniae species and was named as K. pneumoniae HSL4. The structure of the dha cluster which was responsible for the biosynthesis of PDO was analyzed. It is observed that K. pneumoniae HSL4 was tolerant to salt and partly tolerant to acetate and lactate, which will favor industrial applications. Fed-batch fermentation experiments revealed K. pneumoniae HSL4 exhibited an excellent ability to produce PDO with high concentration (80.08 g L-1), productivity (2.22 g L(-1)h(-1)) and conversion (0.435 g g(-1) or 0.53 mol mol(-1)). The metabolic flux profile illuminated that glycerol was consumed rapidly and PDO was accumulated quickly to a high level during the exponential growth phase. This study provided important information for further fermentation and metabolic engineering of PDO production by K. pneumoniae HSL4. |
| 学科主题 | Science & Technology - Other Topics |
| 原文出处 | 2095-9273 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14857]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Zhou, Sheng 2.Li, Lili 3.Perseke, Marleen 4.Huang, Youhua 5.Wei, Jingguang 6.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 7.[Li, Lili] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Sheng,Li, Lili,Perseke, Marleen,et al. Isolation and characterization of a Klebsiella pneumoniae strain from mangrove sediment for efficient biosynthesis of 1,3-propanediol[J]. SCIENCE BULLETIN,2015,60(5):511-521. |
| APA | Zhou, Sheng,Li, Lili,Perseke, Marleen,Huang, Youhua,Wei, Jingguang,&Qin, Qiwei.(2015).Isolation and characterization of a Klebsiella pneumoniae strain from mangrove sediment for efficient biosynthesis of 1,3-propanediol.SCIENCE BULLETIN,60(5),511-521. |
| MLA | Zhou, Sheng,et al."Isolation and characterization of a Klebsiella pneumoniae strain from mangrove sediment for efficient biosynthesis of 1,3-propanediol".SCIENCE BULLETIN 60.5(2015):511-521. |
入库方式： OAI收割
来源：南海海洋研究所
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