ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis
文献类型：期刊论文
| 作者 | Xiang, Zhiming1; Qu, Fufa1; Wang, Fuxuan1; Xiao, Shu1; Jun, Li1; Zhang, Yang1; Yu, Ziniu1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 42期号:2页码:225-232 |
| 关键词 | Crassostrea hongkongensis Mitochondrial pathway ChBax ChBak |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 英文摘要 | Apoptosis has been primarily investigated in mammals, and little is known about apoptosis in mollusks. The proteins Bax and Bak play critical roles in the mitochondria] apoptosis pathway and in determining cell fate. In this study, ChBax and ChBak, homologs of the well-known Bax and Bak proteins, were identified from the oyster Crassostrea hongkongensis. The ChBax/Bak proteins consist of 207/232 amino acids with the typical domains found in BCL-2 family members. ChBax and ChBak mRNA expression were detected in all 8 of the selected oyster tissues and at the different stages of development. Fluorescence microscopy revealed that the full-length proteins of ChBax/Bak were located in the cytoplasm and mitochondrial outer membrane, of HEK293T cells, respectively. Furthermore, both of the genes' expression levels were found to increase in the hemocytes of oysters challenged with pathogens. The over-expression of ChBax or ChBak activates the p53-Luc reporter gene in HEK293T cells in a dose-dependent manner. These results indicate that ChBax and ChBak may play important roles in the mitochondrial apoptotic pathway in oysters. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 原文出处 | 1050-4648 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14872]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Xiang, Zhiming 2.Qu, Fufa 3.Wang, Fuxuan 4.Xiao, Shu 5.Jun, Li 6.Zhang, Yang 7.Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 8.[Qu, Fufa 9.Wang, Fuxuan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, Zhiming,Qu, Fufa,Wang, Fuxuan,et al. ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(2):225-232. |
| APA | Xiang, Zhiming.,Qu, Fufa.,Wang, Fuxuan.,Xiao, Shu.,Jun, Li.,...&Yu, Ziniu.(2015).ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,42(2),225-232. |
| MLA | Xiang, Zhiming,et al."ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 42.2(2015):225-232. |
入库方式： OAI收割
来源：南海海洋研究所
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