Prostaglandin E receptor 4 (PTGER4) involved in host protection against immune challenge in oyster, Crassostrea hongkongensis
文献类型：期刊论文
| 作者 | Qu, Fufa1; Xiang, Zhiming1; Wang, Fuxuan1; Qi, Lin1; Xu, Fengjiao1; Xiao, Shu1; Yu, Ziniu1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 42期号:2页码:316-324 |
| 关键词 | Crassostrea hongkongensis Prostaglandin E receptor 4 (PTGER4) NF-kappa B pathway Innate immunity |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 英文摘要 | Prostaglandin E receptor 4 (PTGER4) is an essential receptor that can detect various physiological and pathological stimuli and has been implicated in a wide variety of biological processes, including the regulation of immune responses, cytokine production, and apoptosis. In this report, the first mollusk PTGER4, referred to as ChPTGER4, was cloned and characterized from the Hong Kong oyster Crassostrea hongkongensis. Its full-length cDNA is 1734 bp in length, including 5'- and 3'-untranslated region (UTRs) of 354 bp and 306 bp, respectively, and an open reading frame (ORF) of 1074 bp. ChPTGER4 comprises 357 amino acids and shares significant homology with its vertebrate homologs. The results of phylogenetic analysis revealed that ChPTGER4 clusters with PTGER4 from the Pacific oyster. In addition, quantitative real-time PCR analysis revealed that ChPTGER4 was constitutively expressed in all tissues examined and that its expression was significantly up-regulated in hemocytes and gills following challenge by pathogens (Vibrio alginolyticus, Staphylococcus haemolyticus and Saccharomyces cerevisiae) and pathogen-associated molecular patterns (PAMPs: lipopolysaccharide (LPS) and peptidoglycan (PGN). Moreover, fluorescence microscopy analysis revealed that ChPTGER4 localized to the membrane, and its overexpression significantly enhanced NF-kappa B reporter gene activation in the HEK293T cell line. In summary, this study provides the first experimental evidence of a functional PTGER4 in mollusks, which suggests its involvement in the innate immune response in oyster. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 原文出处 | 1050-4648 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14873]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Qu, Fufa 2.Xiang, Zhiming 3.Wang, Fuxuan 4.Xu, Fengjiao 5.Xiao, Shu 6.Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresource Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China 7.[Qu, Fufa 8.Wang, Fuxuan 9.Xu, Fengjiao] Univ Chinese Acad Sci, Beijing 100049, Peoples R China 10.[Qi, Lin] Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qu, Fufa,Xiang, Zhiming,Wang, Fuxuan,et al. Prostaglandin E receptor 4 (PTGER4) involved in host protection against immune challenge in oyster, Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(2):316-324. |
| APA | Qu, Fufa.,Xiang, Zhiming.,Wang, Fuxuan.,Qi, Lin.,Xu, Fengjiao.,...&Yu, Ziniu.(2015).Prostaglandin E receptor 4 (PTGER4) involved in host protection against immune challenge in oyster, Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,42(2),316-324. |
| MLA | Qu, Fufa,et al."Prostaglandin E receptor 4 (PTGER4) involved in host protection against immune challenge in oyster, Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 42.2(2015):316-324. |
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来源：南海海洋研究所
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