Establishment of a new cell line from the heart of giant grouper, Epinephelus lanceolatus (Bloch), and its application in toxicology and virus susceptibility
文献类型：期刊论文
| 作者 | Guo, C. Y.1; Huang, Y. H.1; Wei, S. N.1; Ouyang, Z. L.1; Yan, Y.1; Huang, X. H.1; Qin, Q. W.1 |
| 刊名 | JOURNAL OF FISH DISEASES
 |
| 出版日期 | 2015 |
| 卷号 | 38期号:2页码:175-186 |
| 关键词 | cytotoxicity ELGH Epinephelus lanceolatus transgenic manipulation virus sensitivity |
| 通讯作者 | huangxh@scsio.ac.cn ; qinqw@scsio.ac.cn |
| 英文摘要 | A new marine fish cell line, derived from the heart of giant grouper, Epinephelus lanceolatus (Bloch), was established and characterized. The cell line was designated as ELGH and subcultured with more than 60 passages. The ELGH cells were mainly composed of fibroblast-like cells and multiplied well in Leibovitz's L-15 medium supplemented with 10% foetal bovine serum (FBS) at 28 degrees C. Chromosome analysis indicated that the modal chromosome number was 48. The fluorescent signals were detected in ELGH when transfected with green fluorescent protein reporter plasmids. The 50% cytotoxic concentration (CC50) of the extracellular products (ECPs) from Streptococcus iniae and Vibrio alginolyticus E333 on ELGH cells was 60.02 and 12.49gmL(-1), respectively. Moreover, the ELGH cells showed susceptibility to Singapore grouper iridovirus (SGIV), but not to soft-shelled turtle iridovirus (STIV), red-spotted grouper nervous necrosis virus (RGNNV) and spring viremia of carp virus (SVCV), which was demonstrated by the presence of a severe cytopathic effect (CPE) and increased viral titres. In addition, electron microscopy observation showed that abundant virus particles were present in the infected cells. Taken together, our data above provided the potential utility of ELGH cells for transgenic and genetic manipulation, as well as cytotoxicity testing and virus pathogenesis. |
| 学科主题 | Fisheries; Marine & Freshwater Biology; Veterinary Sciences |
| 原文出处 | 0140-7775 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14876]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Guo, C. Y. 2.Huang, Y. H. 3.Wei, S. N. 4.Ouyang, Z. L. 5.Yan, Y. 6.Huang, X. H. 7.Qin, Q. W.] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 8.[Guo, C. Y.] Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, C. Y.,Huang, Y. H.,Wei, S. N.,et al. Establishment of a new cell line from the heart of giant grouper, Epinephelus lanceolatus (Bloch), and its application in toxicology and virus susceptibility[J]. JOURNAL OF FISH DISEASES,2015,38(2):175-186. |
| APA | Guo, C. Y..,Huang, Y. H..,Wei, S. N..,Ouyang, Z. L..,Yan, Y..,...&Qin, Q. W..(2015).Establishment of a new cell line from the heart of giant grouper, Epinephelus lanceolatus (Bloch), and its application in toxicology and virus susceptibility.JOURNAL OF FISH DISEASES,38(2),175-186. |
| MLA | Guo, C. Y.,et al."Establishment of a new cell line from the heart of giant grouper, Epinephelus lanceolatus (Bloch), and its application in toxicology and virus susceptibility".JOURNAL OF FISH DISEASES 38.2(2015):175-186. |
入库方式： OAI收割
来源：南海海洋研究所
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