Preparative Separation of Sulfur-Containing Diketopiperazines from Marine Fungus Cladosporium sp Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode
文献类型：期刊论文
| 作者 | Gu, Binbin1; Zhang, Yanying1; Ding, Lijian1; He, Shan1; Wu, Bin1; Dong, Junde1; Zhu, Peng1; Chen, Juanjuan1; Zhang, Jinrong1; Yan, Xiaojun1 |
| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2015 |
| 卷号 | 13期号:1页码:354-365 |
| 关键词 | Cladosporium high-speed counter-current chromatography marine fungus sulfur-containing diketopiperazines |
| 通讯作者 | gubin21314@gmail.com ; zyy@scsio.ac.cn ; huahua20062008@126.com ; heshan@nbu.edu.cn ; wubin@zju.edu.cn ; dongjunde@vip.163.com ; zhupeng@nbu.edu.cn ; chenjuanjuan@nbu.edu.cn ; zhangjinrong@nbu.edu.cn ; yanxiaojun@nbu.edu.cn |
| 英文摘要 | High-speed counter-current chromatography (HSCCC) was successively applied to the separation of three sulfur-containing diketopiperazines (DKPs) (including two new compounds cladosporin A (1) and cladosporin B (3), and a known compound haematocin (2)) from a marine fungus Cladosporium sp. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at (1:1:1:1, v/v) and (2:1:2:1, v/v), in stepwise elution mode, was used for HSCCC. The preparative HSCCC separation was performed on 300 mg of crude sample yielding 26.7 mg of compound 3 at a purity of over 95%, 53.6 mg of a mixture of compounds 1 and 2, which was further separated by preparative-HPLC yielding 14.3 mg of compound 1 and 25.4 mg of compound 2 each at a purity of over 95%. Their structures were established by spectroscopic methods. The sulfur-containing DKPs suppressed the proliferation of hepatocellular carcinoma cell line HepG2. The present work represents the first application of HSCCC in the efficient preparation of marine fungal natural products. |
| 学科主题 | Pharmacology & Pharmacy |
| 原文出处 | 1660-3397 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14895]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Gu, Binbin 2.He, Shan 3.Chen, Juanjuan 4.Zhang, Jinrong] Ningbo Univ, Sch Marine Sci, Lab Marine Nat Prod, Ningbo 315211, Zhejiang, Peoples R China 5.[Zhang, Yanying 6.Dong, Junde] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 7.[Ding, Lijian] Jinan Univ, Coll Pharm, Guangzhou 510632, Guangdong, Peoples R China 8.[Wu, Bin] Zhejiang Univ, Ocean Coll, Hangzhou 310058, Zhejiang, Peoples R China 9.[Zhu, Peng 10.Yan, Xiaojun] Ningbo Univ, Minist Educ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Binbin,Zhang, Yanying,Ding, Lijian,et al. Preparative Separation of Sulfur-Containing Diketopiperazines from Marine Fungus Cladosporium sp Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode[J]. MARINE DRUGS,2015,13(1):354-365. |
| APA | Gu, Binbin.,Zhang, Yanying.,Ding, Lijian.,He, Shan.,Wu, Bin.,...&Yan, Xiaojun.(2015).Preparative Separation of Sulfur-Containing Diketopiperazines from Marine Fungus Cladosporium sp Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode.MARINE DRUGS,13(1),354-365. |
| MLA | Gu, Binbin,et al."Preparative Separation of Sulfur-Containing Diketopiperazines from Marine Fungus Cladosporium sp Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode".MARINE DRUGS 13.1(2015):354-365. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。