Nuclear factor of activated T cells (NFAT) in pearl oyster Pinctada fucata: Molecular cloning and functional characterization
文献类型：期刊论文
| 作者 | Huang, Xian-De1; Wei, Guo-jian1; Zhang, Hua1; He, Mao-Xian1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 42期号:1页码:108-113 |
| 关键词 | Pearl oyster PfNFAT Gene cloning Gene expression |
| 通讯作者 | hmx@scsio.ac.cn |
| 英文摘要 | Nuclear factor of activated T cells (NFAT) plays an important role in nonimmune cells and also in T cells and many other cells of the immune system, by regulating the expression of a variety of genes involved in the immune response, organ development, developmental apoptosis and angiogenesis. In the present study, the NFAT homology gene, PfNFAT, from the pearl oyster Pinctada fucata was cloned and its genomic structure and promoter were analyzed. PfNFAT encodes a putative protein of 1226 amino acids, and contains a highly conserved Rel homology region (RHR) with DNA-binding specificity, and a regulatory domain (NFAT homology region, NHR) containing a potent transactivation domain (TAD). The PfNFAT gene consists of 12 exons and 11 introns, and its promoter contains potential binding sites for transcription factors such as NF-kappa B (Nuclear factor kappa B), STATx (signal transducer and activator of transcription), AP-1 (activator protein-1) and Sox-5/9 (SRY type HMG box-5/9), MyoD (Myogenic Differentiation Antigen) and IRF (Interferon regulatory factor). Comparison and phylogenetic analysis revealed that PfNFAT shows high identity with other invertebrate NFAT, and clusters with the NFAT5 subgroup. Furthermore, gene expression analysis revealed that PfNFAT is involved in the immune response to lipopolysaccharide (LPS) and Polyinosinic-polycytidylic acid (poly I:C) stimulation and in the nucleus inserting operation. The study of PfNFAT may increase understanding of molluscan innate immunity. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 原文出处 | 1050-4648 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14898]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Huang, Xian-De 2.Wei, Guo-jian 3.Zhang, Hua 4.He, Mao-Xian] Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Xian-De,Wei, Guo-jian,Zhang, Hua,et al. Nuclear factor of activated T cells (NFAT) in pearl oyster Pinctada fucata: Molecular cloning and functional characterization[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(1):108-113. |
| APA | Huang, Xian-De,Wei, Guo-jian,Zhang, Hua,&He, Mao-Xian.(2015).Nuclear factor of activated T cells (NFAT) in pearl oyster Pinctada fucata: Molecular cloning and functional characterization.FISH & SHELLFISH IMMUNOLOGY,42(1),108-113. |
| MLA | Huang, Xian-De,et al."Nuclear factor of activated T cells (NFAT) in pearl oyster Pinctada fucata: Molecular cloning and functional characterization".FISH & SHELLFISH IMMUNOLOGY 42.1(2015):108-113. |
入库方式： OAI收割
来源：南海海洋研究所
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