Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Chen, Ting1; Wong, Nai-Kei1; Jiang, Xiao1; Luo, Xing1; Zhang, Lvping1; Yang, Dan1; Ren, Chunhua1; Hu, Chaoqun1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2015 |
| 卷号 | 42期号:1页码:114-120 |
| 关键词 | Nitric oxide Antimicrobial molecule Vibrio harveyi Litopenaeus vannamei Hepatopanfcreas |
| 通讯作者 | chan1010@scsio.ac.cn ; wongnk@gmail.com ; jiangxiao4367@126.com ; luoxingzky@163.com ; lpzhang@scsio.ac.cn ; yangdan@hku.hk ; rosemary166@sina.com ; hucq@scsio.ac.cn |
| 英文摘要 | Nitric oxide (NO) is a key effector molecule produced in the innate immune systems of many species for antimicrobial defense. However, how NO production is regulated during bacterial infection in invertebrates, especially crustaceans, remains poorly understood. Vibrio harveyi, a Gram-negative marine pathogen, is among the most prevalent and serious threats to the world's shrimp culture industry. Its virulence typically manifests itself through shrimp hepatopancreas destruction. In the current study, we found that NO generated by an in vitro donor system (NOC-18) could rapidly and effectively kill V harveyi. In addition, injection of heat-killed V. harveyi increased the concentration of NO/nitrite and the mRNA expression of nitric oxide synthase (NOS) in the hepatopancreas of Pacific white shrimp (Litopenaeus vannamei), the commercially most significant shrimp species. Live V. harveyi challenge also induced NO/nitrite production and NOS gene expression in primary L. vannamei hepatopancreatic cells in a time- and dose-dependent manner. Co-incubation of L-NAME, an inhibitor selective for mammalian constitutive NOSs, dose-dependently blocked V harveyi-induced NO/nitrite production, without affecting V harveyi-induced NOS mRNA expression. Furthermore, L-NAME treatment significantly increased the survival rate of infecting V harveyi in cultured primary hepatopancreatic cells of L. vannamei. As a whole, we have demonstrated that endogenous NO produced by L. vannamei hepatopancreatic cells occurs in enzymatically regulated manners and is sufficient to act as a bactericidal molecule for V. harveyi clearance. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 原文出处 | 1050-4648 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/14899]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.[Chen, Ting 2.Jiang, Xiao 3.Luo, Xing 4.Zhang, Lvping 5.Ren, Chunhua 6.Hu, Chaoqun] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Key Lab Appl Marine Biol Guangdong Prov, South China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China 7.[Wong, Nai-Kei 8.Yang, Dan] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China 9.[Luo, Xing] Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Ting,Wong, Nai-Kei,Jiang, Xiao,et al. Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(1):114-120. |
| APA | Chen, Ting.,Wong, Nai-Kei.,Jiang, Xiao.,Luo, Xing.,Zhang, Lvping.,...&Hu, Chaoqun.(2015).Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,42(1),114-120. |
| MLA | Chen, Ting,et al."Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 42.1(2015):114-120. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。