c-Jun N-terminal kinases 3 (JNK3) from orange-spotted grouper, Epinephelus coioides, inhibiting the replication of Singapore grouper iridovirus (SGIV) and SGIV-induced apoptosis
文献类型：期刊论文
| 作者 | Guo, Minglan; Wei, Jingguang; Zhou, Yongcan; Qin, Qiwei |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 65页码:169-181 |
| 关键词 | Epinephelus coioides c-Jun N-terminal kinases 3 Singapore grouper iridovirus (SGIV) Apoptosis Antiviral activity |
| 通讯作者 | Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China. |
| 英文摘要 | C-Jun N-terminal kinases (JNKs), a subgroup of serine-threonine protein kinases that activated by phosphorylation, are involve in physiological and pathophysiological processes. JNK3 is one of JNK proteins involved in JNK3 signaling transduction. In the present study, two JNK3 isoforms, Ec-JNK3 X1 and Ec-JNK3 X2, were cloned from orange-spotted grouper, Epinephelus coioides. Both Ec-JNK3 X1 and Ec-JNK3 X2 were mainly expressed in liver, gill, skin, brain and muscle of juvenile grouper. The relative expression of EcANK3 X2 mRNA was much higher in muscle and gill than that of Ec-JNK3 X1. Isoform-specific immune response to challenges was revealed by the expression profiles in vivo. Immunofluorescence staining indicated that JNK3 was localized in the cytoplasm of grouper spleen (GS) cells and shown immune response to SGIV infection in vitro. Over-expressing Ec-JNK3 X1 and/or Ec-JNK3 X2 inhibited the SGIV infection and replication and the SGIV-induced apoptosis. To achieve the antiviral and anti-apoptosis activities, JNK3 promoted the activation of genes ISRE and type I IFN in the antiviral IFN signaling pathway, and inhibited the activation of transcription factors NF-kappa B and p53 relating to apoptosis, respectively. Ec-JNK3 X2 showed stronger activities in antivirus and anti-apoptosis than that of Ec-JNK3 X1. Our results not only define the characterization of JNK3 but also reveal new immune functions and the molecular mechanisms of JNK3 on iridoviruses infection and the virus-induced apoptosis. (C) 2016 Published by Elsevier Ltd. |
| 学科主题 | Immunology; Zoology |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15259]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Guo, Minglan,Wei, Jingguang,Zhou, Yongcan,et al. c-Jun N-terminal kinases 3 (JNK3) from orange-spotted grouper, Epinephelus coioides, inhibiting the replication of Singapore grouper iridovirus (SGIV) and SGIV-induced apoptosis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,65:169-181. |
| APA | Guo, Minglan,Wei, Jingguang,Zhou, Yongcan,&Qin, Qiwei.(2016).c-Jun N-terminal kinases 3 (JNK3) from orange-spotted grouper, Epinephelus coioides, inhibiting the replication of Singapore grouper iridovirus (SGIV) and SGIV-induced apoptosis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,65,169-181. |
| MLA | Guo, Minglan,et al."c-Jun N-terminal kinases 3 (JNK3) from orange-spotted grouper, Epinephelus coioides, inhibiting the replication of Singapore grouper iridovirus (SGIV) and SGIV-induced apoptosis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 65(2016):169-181. |
入库方式： OAI收割
来源：南海海洋研究所
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