Functional Characterization of a Robust Marine Microbial Esterase and Its Utilization in the Stereo-Selective Preparation of Ethyl (S)-3-Hydroxybutyrate
文献类型：期刊论文
| 作者 | Wang, Yilong; Zhang, Yun; Hu, Yunfeng |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 180期号:6页码:1196-1212 |
| 关键词 | Deep-sea microorganism Robust esterase Kinetic resolution Ethyl (S)-3-hydroxybutyrate High stereo-selectivity |
| 通讯作者 | Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China. ; Hu, YF (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China. ; Hu, YF (reprint author), South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China. |
| 英文摘要 | One novel microbial esterase PHE21 was cloned from the genome of Pseudomonas oryzihabitans HUP022 identified from the deep sea of the Western Pacific. PHE21 was heterologously expressed and functionally characterized to be a robust esterase which behaved high resistance to various metal ions, organic solvents, surfactants, and NaCl. Despite the fact that the two enantiomers of ethyl 3-hydroxybutyrate were hard to be enzymatically resolved before, we successfully resolved racemic ethyl 3-hydroxybutyrate through direct hydrolysis reactions and generated chiral ethyl (S)-3-hydroxybutyrate using esterase PHE21. After process optimization, the enantiomeric excess, the conversion rate, and the yield of desired product ethyl (S)-3-hydroxybutyrate could reach 99, 65, and 87 %, respectively. PHE21 is a novel marine microbial esterase with great potential in asymmetric synthesis as well as in other industries. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15262]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Yilong,Zhang, Yun,Hu, Yunfeng. Functional Characterization of a Robust Marine Microbial Esterase and Its Utilization in the Stereo-Selective Preparation of Ethyl (S)-3-Hydroxybutyrate[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,180(6):1196-1212. |
| APA | Wang, Yilong,Zhang, Yun,&Hu, Yunfeng.(2016).Functional Characterization of a Robust Marine Microbial Esterase and Its Utilization in the Stereo-Selective Preparation of Ethyl (S)-3-Hydroxybutyrate.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,180(6),1196-1212. |
| MLA | Wang, Yilong,et al."Functional Characterization of a Robust Marine Microbial Esterase and Its Utilization in the Stereo-Selective Preparation of Ethyl (S)-3-Hydroxybutyrate".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 180.6(2016):1196-1212. |
入库方式： OAI收割
来源：南海海洋研究所
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