Antiviral function of Tachyplesin I against iridovirus and nodavirus
文献类型：期刊论文
| 作者 | Xie, Haiwei; Wei, Jingguang; Qin, Qiwei |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 58页码:96-102 |
| 关键词 | Tachyplesin I Antiviral effects Epinephelus coioides SGIV RGNNV |
| 通讯作者 | Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China. |
| 英文摘要 | Antimicrobial peptides (AMPs) are ubiquitously found in living organisms and are an important component in innate immune response. Tachyplesin I is a potent antimicrobial peptide isolated from the hemocytes of the horseshoe crab, Tachypleus tridentatus. Previous studies have shown that the 17-residue peptide exhibits a wide spectrum of antimicrobial activity against Gram-negative and Grampositive bacteria, fungi, protozoa, and viruses. However, the efficiencies and defense mechanisms of the Tachyplesin I against fish viruses are still unknown. In this study, Tachyplesin I showed a key role in inhibiting the infection and replication of two kinds of newly emerging marine fish viruses, an enveloped DNA virus of Singapore grouper iridovirus (SGIV), and a non-enveloped RNA virus of viral nervous necrosis virus (RGNNV). Synthetic peptides of Tachyplesin I incubated with virus or cells before infection reduced the viral infectivity. Synthetic peptides of Tachyplesin I drastically decreased SGIV and RGNNV titers and viral gene expression. Grouper spleen (GS) and brain (GB) cells over-expressing Tachyplesin I (GS/pcDNA3.1-flag-Tac I and GB/pcDNA3.1-flag-Tac I) support the inhibition of viral infection. Tachyplesin I activated type I IFN and Interferon-sensitive response element (ISRE) in vitro. The promoter activity of IFN-13 and ISRE were significantly up-regulated in cells transfected with pcDNA3.1-flag-Tac I after infection with SGIV and VNNV. These results suggest that Tachyplesin I is importantly involved in host immune responses to invasion of viral pathogens. (C) 2016 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15264]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Xie, Haiwei,Wei, Jingguang,Qin, Qiwei. Antiviral function of Tachyplesin I against iridovirus and nodavirus[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58:96-102. |
| APA | Xie, Haiwei,Wei, Jingguang,&Qin, Qiwei.(2016).Antiviral function of Tachyplesin I against iridovirus and nodavirus.FISH & SHELLFISH IMMUNOLOGY,58,96-102. |
| MLA | Xie, Haiwei,et al."Antiviral function of Tachyplesin I against iridovirus and nodavirus".FISH & SHELLFISH IMMUNOLOGY 58(2016):96-102. |
入库方式： OAI收割
来源：南海海洋研究所
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