Negative regulation of the innate antiviral immune response by TRIM62 from orange spotted grouper
文献类型：期刊论文
| 作者 | Yang, Ying; Huang, Youhua; Yu, Yepin; Zhou, Sheng; Wang, Shaowen; Yang, Min; Qin, Qiwei; Huang, Xiaohong |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 57页码:68-78 |
| 关键词 | TRIM62 Grouper RGNNV Antiviral Interferon |
| 通讯作者 | Qin, QW ; Huang, XH (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China. |
| 英文摘要 | Increased reports uncovered that mammalian tripartite motif-containing 62 (TRIM62) exerts crucial roles in cancer and innate immune response. However, the roles of fish TRIM62 in antiviral immune response remained uncertain. In this study, a TRIM62 gene was cloned from orange spotted grouper (EcTRIM62) and its roles in grouper RNA virus infection was elucidated in vitro. EcTRIM62 shared 99% and 83% identity to bicolor damselfish (Stegastes partitus) and human (Homo sapiens), respectively. Sequence alignment indicated that EcTRIM62 contained three domains, including a RING-finger domain, a B-box domain and a SPRY domain. In healthy grouper, the transcript of EcTRIM62 was predominantly detected in brain and liver, followed by heart, skin, spleen, fin, gill, intestine, and stomach. Subcellular localization analysis indicated that bright fluorescence spots were observed in the cytoplasm of EcTRIM62-transfected grouper spleen (GS) cells. During red-spotted grouper nervous necrosis (RGNNV) infection, overexpression of EcTRIM62 significantly enhanced the severity of CPE and increased viral gene transcriptions. Furthermore, the ectopic expression of EcTRIM62 significantly decreased the transcription level of interferon signaling molecules, including interferon regulatory factor 3 (IRF3), IRF7, interferon stimulated gene 15 (ISG15), melanoma differentiation-associated protein 5 (MDA5), myxovirus resistance gene MXI, and MXII, suggesting that the negative regulation of interferon immune response by EcTRIM62 might directly contributed to its enhancing effect on RGNNV replication. Furthermore, our results also demonstrated that overexpression of EcTRIM62 was able to differently regulate the expression levels of pro-inflammation cytokines. In addition, we found the ectopic expression of EcTIRM62 negatively regulated MDA5-, but not mediator of IRF3 activation (MITA)-induced interferon immune response. Further studies showed that the deletion of RING domain and SPRY domain significantly affected the action of EcTRIM62, including the enhancing effect on virus replication and regulation of interferon immune response. Thus, our studies firstly demonstrated that EcTRIM62 negatively regulated the innate antiviral immune response against fish RNA viruses. (C) 2016 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15286]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang, Ying,Huang, Youhua,Yu, Yepin,et al. Negative regulation of the innate antiviral immune response by TRIM62 from orange spotted grouper[J]. FISH & SHELLFISH IMMUNOLOGY,2016,57:68-78. |
| APA | Yang, Ying.,Huang, Youhua.,Yu, Yepin.,Zhou, Sheng.,Wang, Shaowen.,...&Huang, Xiaohong.(2016).Negative regulation of the innate antiviral immune response by TRIM62 from orange spotted grouper.FISH & SHELLFISH IMMUNOLOGY,57,68-78. |
| MLA | Yang, Ying,et al."Negative regulation of the innate antiviral immune response by TRIM62 from orange spotted grouper".FISH & SHELLFISH IMMUNOLOGY 57(2016):68-78. |
入库方式： OAI收割
来源：南海海洋研究所
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