MKK7 confers different activities to viral infection of Singapore grouper iridovirus (SGIV) and nervous necrosis virus (NNV) in grouper
文献类型：期刊论文
| 作者 | Guo, Minglan; Wei, Jingguang; Zhou, Yongcan; Qin, Qiwei |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 57页码:419-427 |
| 关键词 | Epinephelus coioides MKK7 Molecular characterization Singapore grouper iridovirus (SGIV) Nervous necrosis virus (NNV) |
| 通讯作者 | Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China. ; Zhou, YC (reprint author), Hainan Univ, Coll Marine Sci, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Peoples R China. |
| 英文摘要 | Mitogen-activated protein kinase 7 (MKK7) is one of the major stress-activated protein kinase (SAPK)activating kinases in response to environmental or physiological stimuli. Here a MKK7 named as Ec-MKK7 was identified from orange-spotted grouper, Epinephelus coioides. The full-length cDNA of Ec-MKK7 was 1853 bp, with an open reading frame (ORF) of 1272 bp encoding a putative protein of 423 amino acids. A characteristic S-K-A-K-T motif was contained in the domain of dual-specificity protein kinase, mitogen-activated protein kinase kinase 7 (PKc_MKK7). Intracellular localization showed that Ec-MKK7 was localized in both the cytoplasm and the nucleus of grouper spleen (GS) and/or grouper brain (EAGB) cells. Moreover, Ec-MKK7 was universally expressed in all examined tissues and showed expression modulation to challenges of lipopolysacchride (LPS), Singapore grouper iridovirus (SGIV) and polyriboinosinic polyribocytidylic acid (poly I:C) in vivo. A gene targeting strategy over-expressing Ec-MKK7 was performed to examine the activities of MKK7 to viral infection in vitro. Our data showed that Ec-MKK7 was involved in the evasion and replication of SGIV but played an antiviral role to the infection of nervous necrosis virus (NNV). All results demonstrated that Ec-MKK7 could play important roles in grouper innate immunity and show distinct functions on virus infection. (C) 2016 ElSevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15287]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Guo, Minglan,Wei, Jingguang,Zhou, Yongcan,et al. MKK7 confers different activities to viral infection of Singapore grouper iridovirus (SGIV) and nervous necrosis virus (NNV) in grouper[J]. FISH & SHELLFISH IMMUNOLOGY,2016,57:419-427. |
| APA | Guo, Minglan,Wei, Jingguang,Zhou, Yongcan,&Qin, Qiwei.(2016).MKK7 confers different activities to viral infection of Singapore grouper iridovirus (SGIV) and nervous necrosis virus (NNV) in grouper.FISH & SHELLFISH IMMUNOLOGY,57,419-427. |
| MLA | Guo, Minglan,et al."MKK7 confers different activities to viral infection of Singapore grouper iridovirus (SGIV) and nervous necrosis virus (NNV) in grouper".FISH & SHELLFISH IMMUNOLOGY 57(2016):419-427. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。