The first molluscan TRIM9 is involved in the negative regulation of NF-kappa B activity in the Hong Kong oyster, Crassostrea hongkongensis
文献类型：期刊论文
| 作者 | Liu, Ying; Li, Jun; Wang, Fuxuan; Mao, Fan; Zhang, Yuehuan; Zhang, Yang; Yu, Ziniu |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 56页码:106-110 |
| 关键词 | Crassostrea hongkongensis TRIM9 Innate immunity NF-kappa B |
| 通讯作者 | Yu, ZN (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China. |
| 英文摘要 | TRIM proteins are a group of highly conserved proteins participating in a variety of biological processes such as regulation of development, apoptosis, and innate immunity. However, the functions of these proteins in the mollusk are still poorly understood. In the present study, a TRIM9 homolog (named ChTRIM9) was first identified from a transcript-ome library in the Hong Kong oyster Crassostrea hongkongensis. The full-length cDNA of ChTRIM9 is 2928 bp and has a predicted Open Reading Frame ORF) encoding 721 amino acids, encoding a putative 80.2 kDa protein. SMART analysis indicated that ChTRIM9 contains the three typical TRIM domains, a RING finger, two B-boxes, and a coiled-coil domain in the N-terminal region, whereas the C-terminal region contains a SPRY domain. qRT-PCR,analysis revealed a ubiquitous presence of ChTRIM9, with the highest expression in the gills. Upon bacterial challenge in vivo, the ChTRIM9 transcripts in hemocytes were significantly down-regulated, indicating its involvement in signal transduction in immune response of oysters. Furthermore, ChTRIM9 was found to be localized mainly in the cytoplasm, and its over-expression inhibited the transcriptional activity of the NF-kappa B gene in HEK293T cells, demonstrating its negative role in regulating NF-kappa B signaling. (C) 2016 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15297]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, Ying,Li, Jun,Wang, Fuxuan,et al. The first molluscan TRIM9 is involved in the negative regulation of NF-kappa B activity in the Hong Kong oyster, Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2016,56:106-110. |
| APA | Liu, Ying.,Li, Jun.,Wang, Fuxuan.,Mao, Fan.,Zhang, Yuehuan.,...&Yu, Ziniu.(2016).The first molluscan TRIM9 is involved in the negative regulation of NF-kappa B activity in the Hong Kong oyster, Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,56,106-110. |
| MLA | Liu, Ying,et al."The first molluscan TRIM9 is involved in the negative regulation of NF-kappa B activity in the Hong Kong oyster, Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 56(2016):106-110. |
入库方式： OAI收割
来源：南海海洋研究所
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