MHC class II alpha polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides
文献类型：期刊论文
| 作者 | Yang, Min; Wei, Jingguang; Li, Pengfei; Wei, Shina; Huang, Youhua; Qin, Qiwei |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2016 |
| 卷号 | 462页码:2016-10-16 |
| 关键词 | Disease resistance Major histocompatibility complex II alpha Orange-spotted grouper Selective breeding program Singapore grouper iridovirus |
| 通讯作者 | Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China. |
| 英文摘要 | The major histocompatibility complex (MHC) is a crucial component of the immune systems of vertebrate animals and is associated with their resistance/susceptibility to pathogenic diseases. In this study, the association between MHC II alpha gene polymorphisms and disease resistance/susceptibility was analyzed in the orange-spotted grouper (Epinephelus coioides), by challenging it with Singapore grouper iridovirus (SGIV). Thirty-seven alleles showing high levels of polymorphism (24.7% mutation sites) were isolated from 80 individuals. In the peptide-binding region, the ratio of nonsynonymous (dN) substitutions to synonymous (dS) substitutions was 1.427 (>1), suggesting that the loci are evolving under positive balancing selection. Eight alleles were selected to estimate their distributions in high-resistance (HR) and high-susceptibility (HS) groups of fish. The EPCO-DAA*0101 allelewas significantly associated with susceptibility to SGIV infection (P < 0.05) and the EPCO-DAA*0104, EPCO-DAA* 0601, EPCO-DAA*1101, and EPCO-DAA*1201 alleles were significantly associated with resistance to SGIV (P < 0.05 for each). To confirm these correlations, an additional independent challenge experiment was performed in the Wenchang population of the orange-spotted grouper. The frequency distribution showed that the EPCO-DAA*0101 allele was significantly more frequent in the Wenchang HS (WC-HS) group than in the WC-HR group (P < 0.05), whereas the EPCO-DAA*1101 allele was significantly more frequent in the WC-HR group than in the WC-HS group (P < 0.05), consistent with the first challenge. However, the frequency distributions of the EPCO-DAA*0104, EPCO-DAA*0601, and EPCO-DAA*1201 alleles did not differ significantly between the WC-HR and WC-HS groups (P > 0.05). These results indicate that the EPCO-DAA*0101 allele confers susceptibility to SGIV infection, whereas the EPCO-DAA*1101 allele confers resistance to it. These disease-resistance- related MHC markers could be useful for the molecular-marker-assisted selective breeding of the orange-spotted grouper. (C) 2016 Elsevier B.V. All rights reserved. |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15304]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang, Min,Wei, Jingguang,Li, Pengfei,et al. MHC class II alpha polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides[J]. AQUACULTURE,2016,462:2016-10-16. |
| APA | Yang, Min,Wei, Jingguang,Li, Pengfei,Wei, Shina,Huang, Youhua,&Qin, Qiwei.(2016).MHC class II alpha polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides.AQUACULTURE,462,2016-10-16. |
| MLA | Yang, Min,et al."MHC class II alpha polymorphisms and their association with resistance/susceptibility to Singapore grouper iridovirus (SGIV) in orange-spotted grouper, Epinephelus coioides".AQUACULTURE 462(2016):2016-10-16. |
入库方式： OAI收割
来源：南海海洋研究所
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