Cloning, Expression, and Characterization of a Cold-Active and Organic Solvent-Tolerant Lipase from Aeromicrobium sp SCSIO 25071
文献类型：期刊论文
| 作者 | Su, Hongfei; Mai, Zhimao; Yang, Jian; Xiao, Yunzhu; Tian, Xinpeng; Zhang, Si |
| 刊名 | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 26期号:6页码:1067-1076 |
| 关键词 | Lipase cold-active solvent-tolerant Aeromicrobium sp SCSIO 25071 |
| 通讯作者 | Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China. |
| 中文摘要 | The gene encoding lipase (Lip98) from Aeromicrobium sp. SCSIO 25071 was cloned and functionally expressed in Escherichia coli. Lip98 amino acid sequence shares the highest (49%) identity to Rhodococcus jostii RHA1 lipase and contains a novel motif (GHSEG), which is different from other clusters in the lipase superfamily. The recombinant lipase was purified to homogeneity with Ni-NTA affinity chromatography. Lip98 showed an apparent molecular mass of 30 kDa on SDS gel. The optimal temperature and pH value for enzymatic activity were recorded at 30Q degrees C and 7.5, respectively. Lip98 exhibited high activity at low temperatures with 35% maximum activity at 0 degrees C and good stability at temperatures below 35 degrees C. Its calculated activation energy was 4.12 kcal/mol at the low temperature range of 15-30 degrees C. Its activity was slightly affected by some metal ions such as K+, Ca2+, and Na+. The activity of Lip98 was increased by various organic solvents such as DMSO, ethanol, acetone, and hexane with the concentration of 30% (v/v) and retained more than 30% residual activity in neat organic solvent. The unique characteristics of Lip98 imply that it is a promising candidate for industrial application as a nonaqueous biocatalyst and food additive. |
| 学科主题 | Biotechnology & Applied Microbiology; Microbiology |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15403]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Su, Hongfei,Mai, Zhimao,Yang, Jian,et al. Cloning, Expression, and Characterization of a Cold-Active and Organic Solvent-Tolerant Lipase from Aeromicrobium sp SCSIO 25071[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2016,26(6):1067-1076. |
| APA | Su, Hongfei,Mai, Zhimao,Yang, Jian,Xiao, Yunzhu,Tian, Xinpeng,&Zhang, Si.(2016).Cloning, Expression, and Characterization of a Cold-Active and Organic Solvent-Tolerant Lipase from Aeromicrobium sp SCSIO 25071.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,26(6),1067-1076. |
| MLA | Su, Hongfei,et al."Cloning, Expression, and Characterization of a Cold-Active and Organic Solvent-Tolerant Lipase from Aeromicrobium sp SCSIO 25071".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 26.6(2016):1067-1076. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。