Establishment and characterization of a novel cell line from the brain of golden pompano (Trachinotus ovatus)
文献类型：期刊论文
| 作者 | Li, Pengfei; Zhou, Lingli; Ni, Songwei; Xu, Meng; Yu, Yepin; Cai, Jia; Wei, Shina; Qin, Qiwei |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
 |
| 出版日期 | 2016 |
| 卷号 | 52期号:4页码:410-418 |
| 关键词 | Trachinotus ovatus Brain cell line Transfection efficiency Virus susceptibility |
| 通讯作者 | Wei, SN ; Qin, QW (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China. |
| 中文摘要 | Golden pompano is a commercially important marine fish that is widely cultured in China, Japan, and Southeast Asian countries but has been seriously threatened by pathogen. A novel cell line (TOGB) derived from the brain of golden pompano Trachinotus ovatus was established and characterized in this study. TOGB cell line showed high virus susceptibility, especially grouper nervous necrosis virus (GNNV) and Singapore grouper iridovirus (SGIV). As one of the most devastating viruses in marine fish aquaculture, nervous necrosis virus (NNV) causes high mortality rates exceeding 95% in severe outbreaks. Then, TOGB cell line was a useful tool for propagating viruses and provides a potentially valuable resource for the study of viral pathogenesis, the development of antiviral strategies. The TOGB cell lines showed potential application in environmental monitoring. The extracellular products from Vibrio anguillarum and Vibrio alginolyticus demonstrated cytotoxic effects in TOGB cells. TOGB cells grew most rapidly at 28A degrees C, with an optimal concentration of 10% fetal bovine serum in L-15 medium. TOGB cells were diploid (2N = 54). The transfection efficiencies of TOGB cells were 8.6% at the 15th passage and 64.8% at the 45th passage, indicating that the cells are suitable for foreign gene expression. |
| 学科主题 | Cell Biology; Developmental Biology |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15468]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, Pengfei,Zhou, Lingli,Ni, Songwei,et al. Establishment and characterization of a novel cell line from the brain of golden pompano (Trachinotus ovatus)[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2016,52(4):410-418. |
| APA | Li, Pengfei.,Zhou, Lingli.,Ni, Songwei.,Xu, Meng.,Yu, Yepin.,...&Qin, Qiwei.(2016).Establishment and characterization of a novel cell line from the brain of golden pompano (Trachinotus ovatus).IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,52(4),410-418. |
| MLA | Li, Pengfei,et al."Establishment and characterization of a novel cell line from the brain of golden pompano (Trachinotus ovatus)".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 52.4(2016):410-418. |
入库方式： OAI收割
来源：南海海洋研究所
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