Mas5, a homologue of bacterial DnaJ, is indispensable for the host infection and environmental adaptation of a filamentous fungal insect pathogen
文献类型：期刊论文
| 作者 | Wang, Jie; Ying, Sheng-Hua; Hu, Yue; Feng, Ming-Guang |
| 刊名 | ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2016 |
| 卷号 | 18期号:3页码:1037-1047 |
| 通讯作者 | Feng, MG (reprint author), Zhejiang Univ, Inst Microbiol, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China. |
| 中文摘要 | Mas5, a yeast heat shock protein classified to the Hsp40 group, is homologous to bacterial archetype DnaJ but functionally unexplored in filamentous fungi. Here we identify a Mas5 homologue (46.86kDa) in Beauveria bassiana and show its indispensability for host infection and environmental adaptation of the fungal insect pathogen. The deletion of mas5 caused severe defects in aerial conidiation, conidial germination and submerged blastospore production (mimic to host haemocoel). The deletion mutant lost 100% virulence to Galleria mellonella larvae through normal cuticular penetration (topical inoculation) and 50% through cuticle-bypassing infection (intrahaemocoel injection). It formed no blastospore in vivo after inoculation or only a very few after injection. Its extracellular (cuticle degrading) enzymes and virulence-relating Pr1 proteases were 62% and 32% less active respectively. It became more sensitive to high osmolarity, oxidation, cell-wall perturbation, heat shock and UV-B irradiation. These concurred with reduced contents of intracellular mannitol and trehalose, decreased activities of antioxidant enzymes, impaired cell walls and suppressed transcripts of stress-responsive and virulence-relating genes. All the changes were restored by targeted mas5 complementation. All together, Mas5 is indispensable for the in vitro and in vivo life cycle of B.bassiana by targeting many sets of enzymes/proteins at transcriptional and post-transcriptional levels. |
| 学科主题 | Microbiology |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15484]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Jie,Ying, Sheng-Hua,Hu, Yue,et al. Mas5, a homologue of bacterial DnaJ, is indispensable for the host infection and environmental adaptation of a filamentous fungal insect pathogen[J]. ENVIRONMENTAL MICROBIOLOGY,2016,18(3):1037-1047. |
| APA | Wang, Jie,Ying, Sheng-Hua,Hu, Yue,&Feng, Ming-Guang.(2016).Mas5, a homologue of bacterial DnaJ, is indispensable for the host infection and environmental adaptation of a filamentous fungal insect pathogen.ENVIRONMENTAL MICROBIOLOGY,18(3),1037-1047. |
| MLA | Wang, Jie,et al."Mas5, a homologue of bacterial DnaJ, is indispensable for the host infection and environmental adaptation of a filamentous fungal insect pathogen".ENVIRONMENTAL MICROBIOLOGY 18.3(2016):1037-1047. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。