Flavoenzyme CrmK-mediated substrate recycling in caerulomycin biosynthesis
文献类型：期刊论文
| 作者 | Zhu, Yiguang; Picard, Marie-Eve; Zhang, Qingbo; Barma, Julie; Despres, Xavier Murphy; Mei, Xiangui; Zhang, Liping; Duvignaud, Jean-Baptiste; Couture, Manon; Zhu, Weiming |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2016 |
| 卷号 | 7期号:8页码:4867-4874 |
| 通讯作者 | Zhang, CS (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, CAS Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China. ; Shi, R (reprint author), Univ Laval, IBIS, PROTEO, Dept Biochim Microbiol & Bioinformat, Ville De Quebec, PQ G1V 0A6, Canada. |
| 中文摘要 | Substrate salvage or recycling is common and important for primary metabolism in cells but is rare in secondary metabolism. Herein we report flavoenzyme CrmK-mediated shunt product recycling in the biosynthesis of caerulomycin A (CRM A 1), a 2,2'-bipyridine-containing natural product that is under development as a potent novel immunosuppressive agent. We demonstrated that the alcohol oxidase CrmK, belonging to the family of bicovalent FAD-binding flavoproteins, catalyzed the conversion of an alcohol into a carboxylate via an aldehyde. The CrmK-mediated reactions were not en route to 1 biosynthesis but played an unexpectedly important role by recycling shunt products back to the main pathway of 1. Crystal structures and site-directed mutagenesis studies uncovered key residues for FAD-binding, substrate binding and catalytic activities, enabling the proposal for the CrmK catalytic mechanism. This study provides the first biochemical and structural evidence for flavoenzyme-mediated substrate recycling in secondary metabolism. |
| 学科主题 | Chemistry |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/15519]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhu, Yiguang,Picard, Marie-Eve,Zhang, Qingbo,et al. Flavoenzyme CrmK-mediated substrate recycling in caerulomycin biosynthesis[J]. CHEMICAL SCIENCE,2016,7(8):4867-4874. |
| APA | Zhu, Yiguang.,Picard, Marie-Eve.,Zhang, Qingbo.,Barma, Julie.,Despres, Xavier Murphy.,...&Zhang, Changsheng.(2016).Flavoenzyme CrmK-mediated substrate recycling in caerulomycin biosynthesis.CHEMICAL SCIENCE,7(8),4867-4874. |
| MLA | Zhu, Yiguang,et al."Flavoenzyme CrmK-mediated substrate recycling in caerulomycin biosynthesis".CHEMICAL SCIENCE 7.8(2016):4867-4874. |
入库方式： OAI收割
来源：南海海洋研究所
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