产甲烷分离物中 Clostridium spp.与 Methanosarcinabarkeri 潜在的种间直接电子传递
文献类型：期刊论文
| 作者 | 李莹; 郑世玲 ; 张洪霞; 王炳臣; 王欧美; 刘芳华
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| 刊名 | 微生物学通报
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| 出版日期 | 2016-05-24 |
| 页码 | 13 |
| 关键词 | 种间直接电子传递 革兰氏阳性菌 Clostridium spp. Geobacter Methanosarcina barkeri |
| ISSN号 | 0253-2654 |
| 其他题名 | Potential direct interspecies electron transfer (DIET) from Clostridium spp. to Methanosarcina barkeri in amethanogenic isolation |
| 产权排序 | 中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室;中国科学院大学;滨州医学院; |
| 通讯作者 | 王欧美 |
| 中文摘要 | 【目的】革兰氏阴性菌Geobacter metallireducens可以与乙酸型产甲烷菌Methanosaeta harundinacea或Methanosarcina barkeri通过种间直接电子传递(DIET)还原CO2产甲烷。本实验室前期的研究发现Methanosarcina mazei和Geobacteraceae在铁还原富集培养中形成团聚体,可能存在直接电子传递。然而,革兰氏阳性菌(如Clostridium spp.)与产甲烷菌是否存在种间直接电子传递尚不明确。【方法】采用Hungate厌氧滚管法,以乙醇为唯一电子供体从铁还原富集培养体系中获得产甲烷分离物(S6)。通过T-RFL... |
| 英文摘要 | Geobacter metallireducens, a Gram-negative bacterium, can directly transfer electron to acetoclastic methanogens such as Methanosaeta harundinacea and Methanosarcina barkeri for reducing carbon dioxide to methane. Our previous results showed that Methanosarcina mazei and Geobacteraceae formed aggregates in an iron (III)-reducing enrichment culture indicating direct interspecies electron transfer. However, the capability of direct electron transfer with methanogens for Gram-positive iron (III)-reducing bacteria such as Clostridium spp. is still unknown. [Methods] In this further study, a methanogenic isolation (S6) was achieved from the iron (III)-reducing enrichment by roll-tube (Hungate) method with ethanol as the sole electron donor. We used terminal restriction fragment length polymorphism (T-RFLP) and clone library analysis to investigate the community of S6 and used electrochemical method such as cyclic voltammetry (CV) to confirm the electroactivity of isolation. [Results] Clone library analysis of 16S rRNA gene showed that Clostridium spp. (close to C. tunisiense) and Methanosarcina barkeri was predominant in the bacterial and archaeal community, respectively. Interestingly, addition of G. metallireducens into S6 did not increase the ability of both iron (III) reduction and methanogenesis, indicating that Clostridium spp. may play a similar role in direct interspecies electron transfer from G. metallireducens to M. barkeri. Furthermore, current generation of the S6 suspension dramatically decreased when contact between the organisms and the electrodes was prevented by dialysis bag, and CV showed no obvious redox peaks. [Conclusion] These results suggested that there was direct electron transfer in the methanogenic isolation. This work demonstrate that the dominant Gram-positive Clostridium spp. can potentially directly transfer electron to M. barkeri in a methanogenic isolation. |
| 收录类别 | CSCD |
| 语种 | 中文 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/17267]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室 2.中国科学院大学 3.滨州医学院 |
| 推荐引用方式 GB/T 7714 | 李莹,郑世玲,张洪霞,等. 产甲烷分离物中 Clostridium spp.与 Methanosarcinabarkeri 潜在的种间直接电子传递[J]. 微生物学通报,2016:13. |
| APA | 李莹,郑世玲,张洪霞,王炳臣,王欧美,&刘芳华.(2016).产甲烷分离物中 Clostridium spp.与 Methanosarcinabarkeri 潜在的种间直接电子传递.微生物学通报,13. |
| MLA | 李莹,et al."产甲烷分离物中 Clostridium spp.与 Methanosarcinabarkeri 潜在的种间直接电子传递".微生物学通报 (2016):13. |
入库方式： OAI收割
来源：烟台海岸带研究所
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