中国科学院机构知识库网格系统: 土霉素对扇形游仆虫(原生生物)种群生长及遗传多样性的影响

土霉素对扇形游仆虫(原生生物)种群生长及遗传多样性的影响

文献类型：期刊论文


作者	付娆; 龚骏; 张倩倩
刊名	中国科学:生命科学
出版日期	2016-09-20
卷号	46 期号:9 页码:1113-1120
关键词	扇形游仆虫抗生素污染物种群生长单细胞基因组分析遗传多样性
ISSN号	1674-7232
其他题名	Effects of Oxytetracycline on Population Growth and Genetic Diversity of Euplotesvannus (Marine Protist)
产权排序	中国科学院烟台海岸带研究所微生物生态与物质循环实验室;
通讯作者	龚骏，中国科学院烟台海岸带研究所微生物生态与物质循环实验室，E-mail：jgong@yic.ac.cn
中文摘要	抗生素作为一类环境污染物在海岸带生境中广泛存在,它们对海洋原生生物遗传多样性的影响还不清楚.本工作以一种海洋纤毛虫原生生物(扇形游仆虫(Euplotes vannus))为例,研究了一种常见四环类抗生素(土霉素)对其生长的影响,并通过单细胞(个体)分析,探索了抗生素对其大核基因组中核糖体小亚基RNA基因(SSU r DNA)序列多样性的影响.结果表明,与对照组相比,3个土霉素处理组(1,10和20μg/m L)中纤毛虫的自然增长率随土霉素浓度升高显著降低,细胞大小则呈增大趋势.SSU r DNA序列单倍型多样性、核苷酸多样性和GC含量随抗生素浓度升高呈现降低趋势.序列单核苷酸多态性位点的碱基转...
英文摘要	In order to understand the potential impact of antibiotic pollutants on marine protists, the marine ciliate Euplotes vannus was selected as a model organism to test the effects of oxytetracycline (OTC), a tetracycline antibiotic widely used and distributed in coastal environments. The effects of OTC on growth, cell size, and small subunit ribosomal DNA (SSU rDNA) sequence polymorphisms were documented based on single-cell analysis. For the three OTC treatments used (concentrations of 1, 10, and 20 µg/mL), the growth rate decreased significantly with increasing OTC concentrations, whereas cell size showed a tendency of increase, compared with the control. SSU rDNA of single cells generally exhibited lower haplotype and nucleotide diversity, along with slightly lower GC content at higher OTC concentrations. For single nucleotide polymorphisms in SSU rDNA sequences, the frequency of transition was much higher than that of transversion in both control and OTC treatments, although the frequency of transversion increased with increasing dose of OTC. In the treatment with the highest OTC dose, T to C transversion occurred more frequently and A to G reduced substantially, compared with the control. This study indicates that antibiotic pollution has a significant impact on phenotype and physiology of marine ciliates and can lead to changes in the genetic structure of genomic rDNA. It also highlights the high plasticity of protist genomes, which allows these unicellular eukaryotes to rapidly adapt to antibiotic stress.
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:5812037
源URL	[http://ir.yic.ac.cn/handle/133337/17285]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	中国科学院烟台海岸带研究所微生物生态与物质循环实验室
推荐引用方式 GB/T 7714	付娆,龚骏,张倩倩. 土霉素对扇形游仆虫(原生生物)种群生长及遗传多样性的影响[J]. 中国科学:生命科学,2016,46(9):1113-1120.
APA	付娆,龚骏,&张倩倩.(2016).土霉素对扇形游仆虫(原生生物)种群生长及遗传多样性的影响.中国科学:生命科学,46(9),1113-1120.
MLA	付娆,et al."土霉素对扇形游仆虫(原生生物)种群生长及遗传多样性的影响".中国科学:生命科学 46.9(2016):1113-1120.

入库方式： OAI收割

来源：烟台海岸带研究所

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土霉素对扇形游仆虫(原生生物)种群生长及遗传多样性的影响

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