Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1
文献类型：期刊论文
| 作者 | Yin, K; Lv, M ; Wang, QN; Wu, YX; Liao, CY; Zhang, WW; Chen, LX
|
| 刊名 | WATER RESEARCH
 |
| 出版日期 | 2016-10-15 |
| 卷号 | 103页码:383-390 |
| 关键词 | Carboxylesterase E2 Surface display Mercury Bioremediation Biodetection |
| ISSN号 | 0043-1354 |
| 产权排序 | [Yin, Kun; Lv, Min; Wang, Qiaoning; Wu, Yixuan; Zhang, Weiwei; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Liao, Chunyang] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China; [Yin, Kun] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. ; Liao, CY (reprint author), Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China. Email:cyliao@rcees.ac.cn ; lxchen@yic.ac.cn |
| 英文摘要 | Mercury is a toxic heavy metal and presents significant threats to organisms and natural ecosystems. Recently, the mercury remediation as well as its detection by environmental-friendly biotechnology has received increasing attention. In this study, carboxylesterase E2 from mercury-resistant strain Pseudomonas aeruginosa PA1 has been successfully displayed on the outer membrane of Escherichia coli Top10 bacteria to simultaneously adsorb and detect mercury ion (Hg2+). The transmission electron microscopy analysis shows that Hg2+ can be absorbed by carboxylesterase E2 and accumulated on the outer membrane of surface-displayed E. coli bacteria. The adsorption of Hg2+ followed a physicochemical, equilibrated and saturatable mechanism, which well fits the traditional Langmuir adsorption model. The surface-displayed system can be regenerated through regulating pH values. As its activity can be inhibited by Hg2+, carboxylesterase E2 has been used to detect the concentration of Hg2+ in water samples. The developed surface display system will be of great potential in the simultaneous bioremediation and biodetection of environmental mercury pollution. (C) 2016 Elsevier Ltd. All rights reserved. |
| 研究领域[WOS] | Engineering ; Environmental Sciences & Ecology ; Water Resources |
| 关键词[WOS] | HEAVY-METAL IONS ; CELL-SURFACE ; POTENTIAL APPLICATION ; ENZYME BIOSENSOR ; BIOSORPTION ; ADSORPTION ; POLLUTION ; REMOVAL ; DNA ; INHIBITION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000383292100041 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/17203]  |
| 专题 | 烟台海岸带研究所_污染过程与控制实验室 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 推荐引用方式 GB/T 7714 | Yin, K,Lv, M,Wang, QN,et al. Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1[J]. WATER RESEARCH,2016,103:383-390. |
| APA | Yin, K.,Lv, M.,Wang, QN.,Wu, YX.,Liao, CY.,...&Chen, LX.(2016).Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1.WATER RESEARCH,103,383-390. |
| MLA | Yin, K,et al."Simultaneous bioremediation and biodetection of mercury ion through surface display of carboxylesterase E2 from Pseudomonas aeruginosa PA1".WATER RESEARCH 103(2016):383-390. |
入库方式： OAI收割
来源：烟台海岸带研究所
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