Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction
文献类型：期刊论文
| 作者 | Han, XY; Yu, FB; Song, XY; Chen, LX
|
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2016 |
| 卷号 | 7期号:8页码:5098-5107 |
| ISSN号 | 2041-6520 |
| 产权排序 | [Han, Xiaoyue; Yu, Fabiao; Song, Xinyu; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Han, Xiaoyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. Email:lxchen@yic.ac.cn |
| 英文摘要 | Cysteine hydropersulfide (Cys-SSH) plays primary roles in the synthesis of sulfur-containing cofactors, regulation of cellular signaling, activation or inactivation of enzyme activities, and modulation of cellular redox milieu. However, its biofunctions need to be further addressed due to the fact that many issues remain to be clarified. Herein, we conceive a novel ratiometric near-infrared fluorescent probe Cy-Dise for the sensitive and selective detection of Cys-SSH in living cells and in vivo for the first time. Cy-Dise is composed of three moieties: bis(2-hydroxyethyl) diselenide, heptamethine cyanine, and D-galactose. Cy-Dise exhibits a satisfactory linear ratio response to Cys-SSH via a selenium-sulfur exchange reaction in the range of 0-12 mu M Cys-SSH. The experimental detection limit is determined to be 0.12 mu M. The results of ratio imaging analyses confirm the qualitative and quantitative detection capabilities of Cy-Dise in HepG2 cells, HL-7702 cells, and primary hepatocytes. The level changes of Cys-SSH in cells stimulated by some related reagents are also observed. The probe is also suitable for deep tissue ratio imaging. Organ targeting tests with Cy-Dise in normal Spraque-Dawley (SD) rats and Walker-256 tumor SD rats verify its predominant localization in the liver. The probe is promising for revealing the roles of Cys-SSH in physiological and pathological processes. |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | HIGHLY SELECTIVE DETECTION ; PROTEIN S-SULFHYDRATION ; LIVING CELLS ; IN-VIVO ; HYDROGEN POLYSULFIDES ; OXIDATIVE STRESS ; BIOSYNTHETIC PATHWAYS ; TISSUE DISTRIBUTION ; BIOLOGICAL-SYSTEMS ; HYPOCHLOROUS ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000380893900036 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/17221]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 推荐引用方式 GB/T 7714 | Han, XY,Yu, FB,Song, XY,et al. Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction[J]. CHEMICAL SCIENCE,2016,7(8):5098-5107. |
| APA | Han, XY,Yu, FB,Song, XY,&Chen, LX.(2016).Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction.CHEMICAL SCIENCE,7(8),5098-5107. |
| MLA | Han, XY,et al."Quantification of cysteine hydropersulfide with a ratiometric near-infrared fluorescent probe based on selenium-sulfur exchange reaction".CHEMICAL SCIENCE 7.8(2016):5098-5107. |
入库方式： OAI收割
来源：烟台海岸带研究所
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