Chemical Targeting of a G-Quadruplex RNA in the Ebola Virus L Gene
文献类型：期刊论文
| 作者 | Wang, Shao-Ru1; Zhang, Qiu-Yan2; Wang, Jia-Qi1; Ge, Xing-Yi2; Song, Yan-Yan1; Wang, Ya-Fen1; Li, Xiao-Dan2; Fu, Bo-Shi1; Xu, Guo-Hua3; Shu, Bo2 |
| 刊名 | CELL CHEMICAL BIOLOGY
 |
| 出版日期 | 2016-09-22 |
| 卷号 | 23期号:9页码:1113-1122 |
| 英文摘要 | In the present study, our bioinformatics analysis first reveals the existence of a conserved guanine-rich sequence within the Zaire ebolavirus L gene. Using various methods, we show that this sequence tends to fold into G-quadruplex RNA. TMPyP4 treatment evidently inhibits L gene expression at the RNA level. Moreover, the mini-replicon assay demonstrates that TMPyP4 effectively inhibits the artificial Zaire ebolavirus mini-genome and is a more potent inhibitor than ribavirin. Although TMPyP4 treatment reduced the replication of the mutant mini-genome when G-quadruplex formation was abolished in the L gene, its inhibitory effect was significantly alleviated compared with wild-type. Our findings thus provide the first evidence that G-quadruplex RNA is present in a negative-sense RNA virus. Finally, G-quadruplex RNA stabilization may represent a new therapeutic strategy against Ebola virus disease. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | DNA G-QUADRUPLEX ; C-MYC ; HIGH-THROUGHPUT ; SMALL-MOLECULE ; FORMING SEQUENCES ; DRUG DEVELOPMENT ; HUMAN GENOME ; IN-VIVO ; PROMOTER ; TRANSLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000384731600013 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/9594]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Wuhan Univ, Inst Adv Studies, Coll Chem & Mol Sci, Key Lab Biomed Polymers,Minist Educ, Wuhan 430072, Hubei Province, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei Province, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shao-Ru,Zhang, Qiu-Yan,Wang, Jia-Qi,et al. Chemical Targeting of a G-Quadruplex RNA in the Ebola Virus L Gene[J]. CELL CHEMICAL BIOLOGY,2016,23(9):1113-1122. |
| APA | Wang, Shao-Ru.,Zhang, Qiu-Yan.,Wang, Jia-Qi.,Ge, Xing-Yi.,Song, Yan-Yan.,...&Zhou, Xiang.(2016).Chemical Targeting of a G-Quadruplex RNA in the Ebola Virus L Gene.CELL CHEMICAL BIOLOGY,23(9),1113-1122. |
| MLA | Wang, Shao-Ru,et al."Chemical Targeting of a G-Quadruplex RNA in the Ebola Virus L Gene".CELL CHEMICAL BIOLOGY 23.9(2016):1113-1122. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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